Literature summary for 5.3.1.5 extracted from

Lonn, A.; Gardonyi, M.; van Zyl, W.; Hahn-Hagerdal, B.; Otero, R.C.
Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis. Gene cloning and protein characterization (2002), Eur. J. Biochem., 269, 157-163.

Search for more literature for 5.3.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli HB101	Thermus thermophilus

Protein Variants

Protein Variants	Comment	Organism
E372G	broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type	Thermus thermophilus
E372G/F163L	broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type	Thermus thermophilus
E372G/V379A	broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type	Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
xylitol	-	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.44	-	D-xylose	60°C, pH 7, wild-type	Thermus thermophilus
25.1	-	D-xylose	60°C, pH 7, mutant E372G/V379A	Thermus thermophilus
28.7	-	D-xylose	60°C, pH 7, mutant E372G	Thermus thermophilus
52	-	D-glucose	60°C, pH 7, mutant E372G/V379A	Thermus thermophilus
89.4	-	D-xylose	60°C, pH 7, mutant E372G/F163L	Thermus thermophilus
130.8	-	D-glucose	60°C, pH 7, mutant E372G/F163L	Thermus thermophilus
146.8	-	D-glucose	60°C, pH 7, wild-type	Thermus thermophilus
171.8	-	D-glucose	60°C, pH 7, mutant E372G	Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	-	Thermus thermophilus
Mg2+	-	Thermus thermophilus
Mn2+	-	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glucose	-	Thermus thermophilus	D-Fructose	-	r
D-Xylose	-	Thermus thermophilus	D-Xylulose	-	r

Synonyms

Synonyms	Comment	Organism
XI	-	Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	mutants E372G/V379A	Thermus thermophilus
90	-	wild-type	Thermus thermophilus
95	-	mutants E372G/F163L and E372G	Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.7	-	D-glucose	60°C, pH 7, mutant E372G/F163L	Thermus thermophilus
0.833	-	D-glucose	60°C, pH 7, wild-type	Thermus thermophilus
16.3	-	D-glucose	60°C, pH 7, wild-type	Thermus thermophilus
23.8	-	D-glucose	60°C, pH 7, mutant E372G	Thermus thermophilus
39.9	-	D-glucose	60°C, pH 7, mutant E372G/V379A	Thermus thermophilus
46.6	-	D-xylose	60°C, pH 7, wild-type	Thermus thermophilus
66.9	-	D-glucose	60°C, pH 7, mutant E372G/F163L	Thermus thermophilus
70.8	-	D-xylose	60°C, pH 7, mutant E372G/F163L	Thermus thermophilus
88.7	-	D-glucose	60°C, pH 7, mutant E372G	Thermus thermophilus
257.5	-	D-xylose	60°C, pH 7, mutant E372G/V379A	Thermus thermophilus
258	-	D-xylose	60°C, pH 7, mutant E372G/V379A	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Thermus thermophilus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	10	mutants E372G/V379A, E372G/F163L and E372G	Thermus thermophilus
5	8	wild-type	Thermus thermophilus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4.6	-	xylitol	60°C, pH 7, wild-type	Thermus thermophilus
33.2	-	xylitol	60°C, pH 7, mutant E372G/V379A	Thermus thermophilus
68.7	-	xylitol	60°C, pH 7, mutant E372G	Thermus thermophilus
1174	-	xylitol	60°C, pH 7, mutant E372G/F163L	Thermus thermophilus

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Release 2023.1 (January 2023)