Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, sequence homology with enzymes from other sources | Thermoanaerobacterium thermosulfurigenes |
Protein Variants | Comment | Organism |
---|---|---|
H101F | substitution of His101 by Phe completely abolishes enzyme activity. When His101 is changed to Glu, Gln, Asn, or Asp, approximately 10-16% of wild-type enzyme activity is retained by the mutant enzymes. The His101Gln mutant enzyme is resistant to diethyldicarbonate inhibition which completely inactivates the wild-type enzyme | Thermoanaerobacterium thermosulfurigenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130 | - |
D-glucose | D-glucose, mutant enzyme His71Phe | Thermoanaerobacterium thermosulfurigenes | |
138 | - |
D-glucose | mutant enzyme His101Gln | Thermoanaerobacterium thermosulfurigenes | |
142 | - |
D-glucose | glucose, wild-type enzyme | Thermoanaerobacterium thermosulfurigenes | |
152 | - |
D-glucose | mutant enzyme His152Phe | Thermoanaerobacterium thermosulfurigenes | |
168 | - |
D-glucose | mutant enzyme His101Asn | Thermoanaerobacterium thermosulfurigenes | |
198 | - |
D-glucose | mutant enzyme His101Asp | Thermoanaerobacterium thermosulfurigenes | |
252 | - |
D-glucose | mutant enzyme His101Glu | Thermoanaerobacterium thermosulfurigenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium thermosulfurigenes | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermoanaerobacterium thermosulfurigenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | - |
Thermoanaerobacterium thermosulfurigenes | D-Fructose | - |
? | |
D-Xylose | - |
Thermoanaerobacterium thermosulfurigenes | D-Xylulose | - |
? |