Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.5 extracted from

  • Khire, J.M.; Lachke, A.H.; Srinivasan, M.C.; Vartak, H.G.
    Characterization of the purified extracellular D-xylose isomerase devoid of D-glucose isomerase from Chainia sp. (1990), Appl. Biochem. Biotechnol., 23, 41-52.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline above 5 mM Streptomyces sp.
Ca2+
-
Streptomyces sp.
D-sorbitol competitive Streptomyces sp.
D-xylitol competitive Streptomyces sp.
EDTA above 5 mM Streptomyces sp.
PCMB above 5 mM Streptomyces sp.
Sn2+
-
Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7
-
D-xylose
-
Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Streptomyces sp.
Mg2+ activates Streptomyces sp.
Mn2+ activates Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000 73000 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Streptomyces sp.

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.39
-
-
Streptomyces sp.

Storage Stability

Storage Stability Organism
-15°C, pH 7.5, 5 mM MgSO4, stable for at least 6 months, in absence of Mg2+ loss of activity within 3 weeks Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose no activity Streptomyces sp. D-Fructose
-
?
D-Ribose no activity Streptomyces sp. ?
-
?
D-Xylose
-
Streptomyces sp. D-Xylulose
-
?
L-Arabinose no activity Streptomyces sp. ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
10 min, stable up to Streptomyces sp.
80
-
10 min, 80% loss of activity in absence of metal ions, 50% loss of activity in presence of 1 mM Mg2+, no loss of activity in presence of 1 mM Co2+ Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7: about 40% of maximal activity, pH 10: about 13.5% of maximal activity Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
highest stability at, 60°C, 30 min Streptomyces sp.