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Literature summary for 5.3.1.5 extracted from

  • Sanchez, S.; Smiley, K.L.
    Properties of D-xylose isomerase from Streptomyces albus (1975), Appl. Microbiol., 29, 745-750.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
Mg2+ and Co2+ protect from thermal denaturation Streptomyces albus

Inhibitors

Inhibitors Comment Organism Structure
D-sorbitol competitive against D-glucose, D-xylose, and D-ribose. Co2+ and Mg2+ prevent inhibition Streptomyces albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
86
-
D-glucose
-
Streptomyces albus
153
-
L-arabinose
-
Streptomyces albus
312
-
L-rhamnose
-
Streptomyces albus
350
-
D-ribose
-
Streptomyces albus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the binding sites for Co2+ and Mn2+ are different from each other Streptomyces albus
Co2+ 1.0 mM, partial activation, Km: 0.003 mM Streptomyces albus
Mg2+ the binding sites for Co2+ and Mn2+ are different from each other Streptomyces albus
Mg2+ 1.0 mM, strong activation, Km: 0.03 mM Streptomyces albus

Organism

Organism UniProt Comment Textmining
Streptomyces albus
-
NRRL 5778
-
Streptomyces albus NRRL 5778
-
NRRL 5778
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose
-
Streptomyces albus D-Fructose
-
?
D-Glucose
-
Streptomyces albus NRRL 5778 D-Fructose
-
?
D-Ribose
-
Streptomyces albus ?
-
?
D-Ribose
-
Streptomyces albus NRRL 5778 ?
-
?
D-Xylose
-
Streptomyces albus D-Xylulose
-
?
D-Xylose
-
Streptomyces albus NRRL 5778 D-Xylulose
-
?
L-Arabinose
-
Streptomyces albus ?
-
?
L-Arabinose
-
Streptomyces albus NRRL 5778 ?
-
?
L-Rhamnose
-
Streptomyces albus ?
-
?
L-Rhamnose
-
Streptomyces albus NRRL 5778 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 80
-
Streptomyces albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Streptomyces albus