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Literature summary for 5.3.1.4 extracted from
- Biochemical properties of L-arabinose isomerase from Clostridium hylemonae to produce D-tagatose as a functional sweetener (2018), PLoS ONE, 13, e0196099 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as inclusion bodies | [Clostridium] hylemonae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, complete loss of activity | [Clostridium] hylemonae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.7 | - |
D-galactose | pH 7.5, 35°C | [Clostridium] hylemonae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 110% of initial activity | [Clostridium] hylemonae | |
| Mg2+ | 1 mM, 120% of initial activity | [Clostridium] hylemonae | |
| Mn2+ | 1 mM, 111% of initial activity | [Clostridium] hylemonae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Clostridium] hylemonae | C0C0X3 | - |
- |
| [Clostridium] hylemonae DSM 15053 | C0C0X3 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.44 | - |
substrate D-galactose, pH 7.5, 35°C | [Clostridium] hylemonae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
[Clostridium] hylemonae | D-tagatose | - |
? | |
| D-galactose | - |
[Clostridium] hylemonae DSM 15053 | D-tagatose | - |
? | |
| L-arabinose | - |
[Clostridium] hylemonae | L-ribulose | - |
? | |
| L-arabinose | - |
[Clostridium] hylemonae DSM 15053 | L-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56554, calculated from sequence, x * 57000, SDS-PAGE | [Clostridium] hylemonae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
[Clostridium] hylemonae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
81% residual activity | [Clostridium] hylemonae |
| 90 | - |
38% residual activity | [Clostridium] hylemonae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
150 min, 90% residual activity | [Clostridium] hylemonae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.4 | - |
D-galactose | pH 7.5, 35°C | [Clostridium] hylemonae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
[Clostridium] hylemonae |
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