Any feedback?
Literature summary for 5.3.1.4 extracted from
- Characterization of a F280N variant of L-arabinose isomerase from Geobacillus thermodenitrificans identified as a D-galactose isomerase (2014), Appl. Microbiol. Biotechnol., 98, 9271-9281 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Geobacillus thermodenitrificans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of mutant C450S/N475K | Geobacillus thermodenitrificans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C450S/N475K | mutant catalyzes the isomerization of D-galactose to D-tagatose | Geobacillus thermodenitrificans |
| F280N/C450S/N475K | mutat catalyzes the isomerization of D-galactose to D-tagatose | Geobacillus thermodenitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.328 | - |
D-galactose | mutant F280N/C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans |  |
| 106 | - |
L-arabinose | mutant C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
| 334 | - |
L-arabinose | mutant F280N/C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
| 339 | - |
D-galactose | mutant C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | Q6W9D4 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Geobacillus thermodenitrificans | D-tagatose | - |
? | |
| L-arabinose | - |
Geobacillus thermodenitrificans | L-ribulose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
mutant F280N/C450S/N475K | Geobacillus thermodenitrificans |
| 70 | - |
mutant C450S/N475K | Geobacillus thermodenitrificans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
mutant C450S/N475K, half-life 728 min | Geobacillus thermodenitrificans |
| 65 | - |
mutant F280N/C450S/N475K, half-life 108 min | Geobacillus thermodenitrificans |
| 75 | - |
mutant C450S/N475K, half-life 47.4 min | Geobacillus thermodenitrificans |
| 75 | - |
mutant F280N/C450S/N475K, half-life 13.3 min | Geobacillus thermodenitrificans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.8 | - |
L-arabinose | mutant F280N/C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
| 17.4 | - |
D-galactose | mutant C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
| 104.1 | - |
D-galactose | mutant F280N/C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
| 240.6 | - |
L-arabinose | mutant C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
mutant F280N/C450S/N475K | Geobacillus thermodenitrificans |
| 8.5 | - |
mutant C450S/N475K | Geobacillus thermodenitrificans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
L-arabinose | mutant F280N/C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
| 0.05 | - |
D-galactose | mutant C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
| 0.08 | - |
D-galactose | mutant F280N/C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
| 2.27 | - |
L-arabinose | mutant C450S/N475K, pH 8, 55°C | Geobacillus thermodenitrificans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
