Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-arabitol | competitive inhibitor | Enterococcus faecium | |
L-ribitol | - |
Enterococcus faecium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 5 | D-galactose | at pH 7.0 and 50°C | Enterococcus faecium | |
42 | - |
L-arabinose | at pH 7.0 and 50°C | Enterococcus faecium | |
71 | - |
D-galactose | at pH 7.0 and 40°C | Enterococcus faecium | |
101 | - |
D-galactose | at pH 7.0 and 30°C | Enterococcus faecium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Enterococcus faecium |
187000 | - |
gel filtration | Enterococcus faecium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | Enterococcus faecium | - |
L-ribulose | - |
? | |
L-arabinose | Enterococcus faecium DBFIQ E36 | - |
L-ribulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecium | - |
- |
- |
Enterococcus faecium DBFIQ E36 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, epoxy-activated Sepharose column chromatography, and L-arabitol-agarose column chromatography | Enterococcus faecium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.25 | - |
cell-free extract, at pH 7.0 and 50°C | Enterococcus faecium |
180 | - |
after 720fold purification, at pH 7.0 and 50°C | Enterococcus faecium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | - |
Enterococcus faecium | D-tagatose | - |
? | |
D-galactose | - |
Enterococcus faecium DBFIQ E36 | D-tagatose | - |
? | |
L-arabinose | - |
Enterococcus faecium | L-ribulose | - |
? | |
L-arabinose | - |
Enterococcus faecium DBFIQ E36 | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 2 * 48000, SDS-PAGE | Enterococcus faecium |
Synonyms | Comment | Organism |
---|---|---|
L-AI | - |
Enterococcus faecium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
activity declines over 60°C | Enterococcus faecium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 70 | the enzyme possesses good stability at 45-50°C, with half-life of 10 h at 50°C. The stability strongly decreases in the range 55-70°C | Enterococcus faecium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
70% of maximal activity at pH 4.5 | Enterococcus faecium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | betweem pH 6.0 and 8.5, the enzyme remains stable with maximum stability at pH 7.0-7.5. The stability decreases moderately at alkaline pH. Enzyme retains more than 60% of its activity when incubated 6 h at pH 6.5-8.5 | Enterococcus faecium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7.9 | - |
L-arabitol | at pH 7.0 and 50°C | Enterococcus faecium | |
183 | - |
L-ribitol | at pH 7.0 and 50°C | Enterococcus faecium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Enterococcus faecium | isoelectric focusing | - |
3.8 |