Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.4 extracted from

  • Torres, P.; Manzo, R.; Rubiolo, A.; Batista-Viera, F.; Mammarella, E.
    Purification of an L-arabinose isomerase from Enterococcus faecium DBFIQ E36 employing a biospecific affinity strategy (2014), J. Mol. Catal. B, 102, 99-105.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
L-arabitol competitive inhibitor Enterococcus faecium
L-ribitol
-
Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3 5 D-galactose at pH 7.0 and 50°C Enterococcus faecium
42
-
L-arabinose at pH 7.0 and 50°C Enterococcus faecium
71
-
D-galactose at pH 7.0 and 40°C Enterococcus faecium
101
-
D-galactose at pH 7.0 and 30°C Enterococcus faecium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
2 * 48000, SDS-PAGE Enterococcus faecium
187000
-
gel filtration Enterococcus faecium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Enterococcus faecium
-
L-ribulose
-
?
L-arabinose Enterococcus faecium DBFIQ E36
-
L-ribulose
-
?

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
-
-
Enterococcus faecium DBFIQ E36
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, epoxy-activated Sepharose column chromatography, and L-arabitol-agarose column chromatography Enterococcus faecium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.25
-
cell-free extract, at pH 7.0 and 50°C Enterococcus faecium
180
-
after 720fold purification, at pH 7.0 and 50°C Enterococcus faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
Enterococcus faecium D-tagatose
-
?
D-galactose
-
Enterococcus faecium DBFIQ E36 D-tagatose
-
?
L-arabinose
-
Enterococcus faecium L-ribulose
-
?
L-arabinose
-
Enterococcus faecium DBFIQ E36 L-ribulose
-
?

Subunits

Subunits Comment Organism
homotetramer 2 * 48000, SDS-PAGE Enterococcus faecium

Synonyms

Synonyms Comment Organism
L-AI
-
Enterococcus faecium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
activity declines over 60°C Enterococcus faecium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 70 the enzyme possesses good stability at 45-50°C, with half-life of 10 h at 50°C. The stability strongly decreases in the range 55-70°C Enterococcus faecium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
70% of maximal activity at pH 4.5 Enterococcus faecium

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 betweem pH 6.0 and 8.5, the enzyme remains stable with maximum stability at pH 7.0-7.5. The stability decreases moderately at alkaline pH. Enzyme retains more than 60% of its activity when incubated 6 h at pH 6.5-8.5 Enterococcus faecium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.9
-
L-arabitol at pH 7.0 and 50°C Enterococcus faecium
183
-
L-ribitol at pH 7.0 and 50°C Enterococcus faecium

pI Value

Organism Comment pI Value Maximum pI Value
Enterococcus faecium isoelectric focusing
-
3.8