Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Thermoanaerobacterium saccharolyticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dulcitol | 90% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum | |
erythritol | 42% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum | |
L-arabitol | 11% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum | |
ribitol | 22% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum | |
xylitol | 46% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
122 | - |
D-galactose | in 25 mM Tris-HCl buffer (pH 7.5), at 60°C | Thermoanaerobacterium saccharolyticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.05 mM Co2+, can improve both catalytic activity and thermostability at higher temperatures | Thermoanaerobacterium saccharolyticum | |
Mn2+ | 0.1 mM Mn2+,increases activity to 167.3% and can improve both catalytic activity and thermostability at higher temperatures | Thermoanaerobacterium saccharolyticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52863 | - |
4 * 52863, native enzyme, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
55000 | - |
4 * 55000, recombinant enzyme, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
55343 | - |
4 * 55343, recombinant enzyme, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
55397 | - |
4 * 55397, recombinant enzyme, MALDI-TOF mass spectrometry | Thermoanaerobacterium saccharolyticum |
233000 | - |
gel filtration | Thermoanaerobacterium saccharolyticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fucose | Thermoanaerobacterium saccharolyticum | 221% activity compared to D-galactose | D-fuculose | - |
r | |
D-galactose | Thermoanaerobacterium saccharolyticum | 100% activity | D-tagatose | - |
r | |
L-arabinose | Thermoanaerobacterium saccharolyticum | 244% activity compared to D-galactose | L-ribulose | - |
r | |
L-arabinose | Thermoanaerobacterium saccharolyticum NTOU1 | 244% activity compared to D-galactose | L-ribulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | K7SW59 | - |
- |
Thermoanaerobacterium saccharolyticum NTOU1 | K7SW59 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Thermoanaerobacterium saccharolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Allose | 9% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
D-Allose | 9% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
D-fucose | 221% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | D-fuculose | - |
r | |
D-galactose | 100% activity | Thermoanaerobacterium saccharolyticum | D-tagatose | - |
r | |
D-rhamnose | 28% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
D-Ribose | 7% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
D-Ribose | 7% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
D-sorbose | 31% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
D-Xylose | 14% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
D-Xylose | 14% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
L-arabinose | 244% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | L-ribulose | - |
r | |
L-arabinose | 244% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum NTOU1 | L-ribulose | - |
r | |
additional information | no activity with D-glucose, D-mannose, and L-fucose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
additional information | no activity with D-glucose, D-mannose, and L-fucose | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 55000, recombinant enzyme, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
homotetramer | 4 * 52863, native enzyme, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
homotetramer | 4 * 55343, recombinant enzyme, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
homotetramer | 4 * 55397, recombinant enzyme, MALDI-TOF mass spectrometry | Thermoanaerobacterium saccharolyticum |
Synonyms | Comment | Organism |
---|---|---|
AraA | - |
Thermoanaerobacterium saccharolyticum |
L-AI | - |
Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermoanaerobacterium saccharolyticum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | the activity increases with the increasing temperature (starting with 22% activity at 40°C, 50% activity at 50°C), reaches an optimal activity at 70°C, and then drops with a steep slope down (65% activity at 75°C) to inactivity at 80°C | Thermoanaerobacterium saccharolyticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 75 | the activity is fully retained after 2 h incubation at 55-65°C. In the presence of 0.1 mM Mn2+ and 0.05 mM Co2+, the enzyme can remain stable for 2 h during the incubation at 55-65°C, and the half-life of the enzyme is about 150 and 15 min at 70 and 75°C, respectively | Thermoanaerobacterium saccharolyticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
D-galactose | in 25 mM Tris-HCl buffer (pH 7.5), at 60°C | Thermoanaerobacterium saccharolyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Thermoanaerobacterium saccharolyticum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | very minimal activity when the pH either drops below 5.0 or increases to above 9.5 | Thermoanaerobacterium saccharolyticum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | remains stable after 24 h. The enzyme activity is fully retained at pH 6.5-9.5 after 6 h with about 70% remaining at pH 5.5 and 10.5 | Thermoanaerobacterium saccharolyticum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-galactose | in 25 mM Tris-HCl buffer (pH 7.5), at 60°C | Thermoanaerobacterium saccharolyticum |