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Literature summary for 5.3.1.4 extracted from
- Bioconversion of D-galactose to D-tagatose: continuous packed bed reaction with an immobilized thermostable L-arabinose isomerase and efficient purification by selective microbial degradation (2012), Appl. Microbiol. Biotechnol., 93, 1469-1474.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Thermoanaerobacter mathranii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 23% residual activity at 1 mM | Thermoanaerobacter mathranii |  |
| Ni2+ | 85% residual activity at 1 mM | Thermoanaerobacter mathranii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | improves the enzymatic activity to 121% at 5 mM | Thermoanaerobacter mathranii | |
| Mn2+ | highest activity with Mn2+. The optimum Mn2+ concentration for the free and calcium alginate-immobilized enzyme is 5 mM (132% activity) | Thermoanaerobacter mathranii | |
| additional information | not activated by Mg2+, Fe2+, and Ca2+ | Thermoanaerobacter mathranii | |
| Zn2+ | improves the enzymatic activity to 115% at 5 mM | Thermoanaerobacter mathranii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE | Thermoanaerobacter mathranii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinose | Thermoanaerobacter mathranii | - |
L-ribulose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter mathranii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Thermoanaerobacter mathranii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Thermoanaerobacter mathranii | D-tagatose | 43.9% yield with immobilized enzyme | ? | |
| L-arabinose | - |
Thermoanaerobacter mathranii | L-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Thermoanaerobacter mathranii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
free enzyme | Thermoanaerobacter mathranii |
| 75 | - |
calcium alginate-immobilized enzyme | Thermoanaerobacter mathranii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
calcium alginate-immobilized enzyme | Thermoanaerobacter mathranii |
| 8 | - |
free enzyme | Thermoanaerobacter mathranii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | the activity of the free enzyme remains about 80% between pH 6.5 and 9.0, while the activity remains above 80% between pH 5.5 and 8.0 in case of the immobilized enzyme | Thermoanaerobacter mathranii |
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Release 2023.1 (January 2023)
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