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Literature summary for 5.3.1.4 extracted from

  • Rhimi, M.; Chouayekh, H.; Gouillouard, I.; Maguin, E.; Bejar, S.
    Production of D-tagatose, a low caloric sweetener during milk fermentation using L-arabinose isomerase (2011), Biores. Technol., 102, 3309-3315.
    View publication on PubMed
Search for more literature for 5.3.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
H175N the mutant exhibits faster D-galactose bioconversion compared to the wild type enzyme Geobacillus stearothermophilus
Q268K the mutant shows a pH optimum of 6.0-6.5 and a higher stability at acidic pH compared to the wild type enzyme, the mutant exhibits faster D-galactose bioconversion compared to the wild type enzyme Geobacillus stearothermophilus
Q268K/H175N the mutant exhibits faster D-galactose bioconversion compared to the wild type enzyme Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus US100
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoQ column chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
 Geobacillus stearothermophilus D-tagatose
-
 ?
D-galactose
-
 Geobacillus stearothermophilus US100 D-tagatose
-
 ?

Synonyms

Synonyms Comment Organism
L-AI
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Geobacillus stearothermophilus