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Literature summary for 5.3.1.4 extracted from

  • Zhang, Y.W.; Prabhu, P.; Lee, J.K.
    Immobilization of Bacillus licheniformis L-arabinose isomerase for semi-continuous L-ribulose production (2009), Biosci. Biotechnol. Biochem., 73, 2234-2239.
    View publication on PubMed
Search for more literature for 5.3.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus licheniformis

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme on different matrices, e.g. activated carboxymethylcellulose, Eupergit C, CNBr-activated agarose, chitosan, and alginate, for semi-continous production of L-ribulose, method optimization, overview. 85.1% remaining activity after 8 cycles and 86.4% activity compared to the wild-type enzyme Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
369
-
 L-arabinose pH 7.5, 50°C, free recombinant enzyme Bacillus licheniformis
770
-
 L-arabinose pH 7.5, 50°C, immobilized recombinant enzyme Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Bacillus licheniformis
-
 L-ribulose
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinose
-
 Bacillus licheniformis L-ribulose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 free enzyme Bacillus licheniformis
55 65 immobilized enzyme Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 free enzyme Bacillus licheniformis
8
-
 immobilized enzyme Bacillus licheniformis