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Literature summary for 5.3.1.4 extracted from
- An L-arabinose isomerase from Acidothermus cellulolytics ATCC 43068: cloning, expression, purification, and characterization (2010), Appl. Microbiol. Biotechnol., 86, 1089-1097.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene araA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Acidothermus cellulolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 30% inhibition at 1 mM | Acidothermus cellulolyticus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates and increase thermostability of the enzyme | Acidothermus cellulolyticus | |
| Mn2+ | activates and increase thermostability of the enzyme | Acidothermus cellulolyticus | |
| additional information | metal ions are essential for catalytic activity, but metal ions, such as Mg2+, Ni2+, and Ba2+, are poor activators, while Fe2+, Ca2+, and Zn2+ have no effect on the activity | Acidothermus cellulolyticus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54768 | - |
x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE | Acidothermus cellulolyticus |
| 55000 | - |
x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE | Acidothermus cellulolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinose | Acidothermus cellulolyticus | - |
L-ribulose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidothermus cellulolyticus | A0LT86 | ATCC 43068, gene araA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment, ion exchange chromatography, and gel filtration | Acidothermus cellulolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | conversion yield over 50% after 12 h under optimal conditions | Acidothermus cellulolyticus | D-tagatose | - |
? | |
| L-arabinose | - |
Acidothermus cellulolyticus | L-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE | Acidothermus cellulolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Acidothermus cellulolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
purified recombinant enzyme, completely stable for 2 h | Acidothermus cellulolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Acidothermus cellulolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | over 90% of maximal activity at pH 6.0, maximal activity at pH 7.5 | Acidothermus cellulolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
25°C, purified recombinant enzyme, 80% activity remaining after 12 h, 70% after 24 h | Acidothermus cellulolyticus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Acidothermus cellulolyticus | sequence calculation | - |
5.63 |
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