Application | Comment | Organism |
---|---|---|
synthesis | an Escherichia coli galactose kinase gene knockout strain, which contains the L-arabinose isomerase gene to isomerize D-galactose to D-tagatose, shows a higher conversion yield of tagatose because galactose is not metabolized by endogenous galactose kinase. In whole cells of the galactose kinase knockout strain, the isomerase-catalyzed reaction exhibits an equilibrium shift towards tagatose, producing a tagatose fraction of 68% at 37°C, whereas the purified L-arabinose isomerase gives a tagatose equilibriumfraction of 36% | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
D-tagatose | pH 7.0, 37°C, purified enzyme | Escherichia coli | |
114 | - |
D-tagatose | pH 7.0, 37°C, whole cells | Escherichia coli | |
256 | - |
D-galactose | pH 7.0, 37°C, whole cells | Escherichia coli | |
365 | - |
D-galactose | pH 7.0, 37°C, purified enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | - |
Escherichia coli | D-tagatose | - |
r | |
D-tagatose | - |
Escherichia coli | D-galactose | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
D-tagatose | pH 7.0, 37°C, purified enzyme | Escherichia coli | |
0.82 | - |
D-tagatose | pH 7.0, 37°C, whole cells | Escherichia coli | |
1.02 | - |
D-galactose | pH 7.0, 37°C, purified enzyme | Escherichia coli | |
4.21 | - |
D-galactose | pH 7.0, 37°C, whole cells | Escherichia coli |