Application | Comment | Organism |
---|---|---|
food industry | production of D-tagatose as a low-calorie sugar-substituting sweetener lower pH is preferable for industrial production | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
A408V | site directed mutagenesis | Geobacillus stearothermophilus |
A408V/K475N | site directed mutagenesis | Geobacillus stearothermophilus |
A475N | site directed mutagenesis | Geobacillus stearothermophilus |
D228N | site directed mutagenesis | Geobacillus stearothermophilus |
D228N/D384G/T393S/N428K/K475N | site directed mutagenesis, GSAI 152 | Geobacillus stearothermophilus |
D384G | site directed mutagenesis | Geobacillus stearothermophilus |
G408V | site directed mutagenesis | Geobacillus stearothermophilus |
K475N | site directed mutagenesis | Geobacillus stearothermophilus |
L408V | site directed mutagenesis | Geobacillus stearothermophilus |
N428K | site directed mutagenesis | Geobacillus stearothermophilus |
Q408V | site directed mutagenesis | Geobacillus stearothermophilus |
Q475N | site directed mutagenesis | Geobacillus stearothermophilus |
R408V | site directed mutagenesis | Geobacillus stearothermophilus |
R475N | site directed mutagenesis | Geobacillus stearothermophilus |
T393S | site directed mutagenesis | Geobacillus stearothermophilus |
V322M | site directed mutagenesis | Geobacillus stearothermophilus |
V322M/T393S/A408V | site directed mutagenesis, GSAI 153 | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9S467 | Geobacillus stearothermophilus | - |
Purification (Comment) | Organism |
---|---|
of the recombinant wild type and mutant enzymes | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
D228N mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.029 | - |
R408V mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.043 | - |
wild type, D-galactose as substrate | Geobacillus stearothermophilus |
0.05 | - |
L408V mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.056 | - |
D384G mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.06 | - |
Q408V mutant, at pH 7.5, D-galactose as substrate | Geobacillus stearothermophilus |
0.06 | - |
R408V mutant, at pH 7.5, D-galactose as substrate | Geobacillus stearothermophilus |
0.062 | - |
Q408V mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.11 | - |
T393S mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.26 | - |
N428K mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.27 | - |
V322M mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.37 | - |
G408V mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.37 | - |
Q475N mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.38 | - |
R475N mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.39 | - |
K475N mutant found in GSAI152, D-galactose as substrate | Geobacillus stearothermophilus |
0.39 | - |
K475N mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.48 | - |
A408V mutant, D-galactose as substrate | Geobacillus stearothermophilus |
0.48 | - |
A475N mutant found in GSAI153, D-galactose as substrate | Geobacillus stearothermophilus |
0.49 | - |
GSAI1 152, D-galactose as substrate | Geobacillus stearothermophilus |
0.59 | - |
A408V/K475N mutant found in GSAI152 or GASI 153w D-galactose as substrate | Geobacillus stearothermophilus |
0.74 | - |
GSAI1 153, D-galactose as substrate | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | - |
Geobacillus stearothermophilus | D-tagatose | - |
? | |
L-arabinose | - |
Geobacillus stearothermophilus | L-ribulose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-galactose isomerase | - |
Geobacillus stearothermophilus |
GSAI | Geobacillus stearothermophilus L-arabinose isomease | Geobacillus stearothermophilus |
GSAI 152 | five mutations | Geobacillus stearothermophilus |
GSAI 153 | three mutations | Geobacillus stearothermophilus |
L-arabinose aldose-ketose-isomerase | - |
Geobacillus stearothermophilus |
L-arabinose isomerase | - |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Q408V mutant enzyme with higher isomerization activites than the wild type enzyme | Geobacillus stearothermophilus |
7.5 | - |
R408V mutant enzyme with higher isomerization activites than the wild type enzyme | Geobacillus stearothermophilus |
8.5 | - |
wild type enzyme | Geobacillus stearothermophilus |