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Literature summary for 5.3.1.4 extracted from
- Characterization of an l-arabinose isomerase from the Lactobacillus plantarum NC8 strain showing pronounced stability at acidic pH (2007), FEMS Microbiol. Lett., 277, 260-267.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | production of D-tagatose as a low-calorie sugar-substituting sweetener | Lactiplantibacillus plantarum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lactiplantibacillus plantarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 43.4 | - |
L-arabinose | at 60°C, pH 7.5 | Lactiplantibacillus plantarum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | required for maximal activity and stability | Lactiplantibacillus plantarum | |
| Cu2+ | inhibitory | Lactiplantibacillus plantarum | |
| Mn2+ | required for maximal activity and stability | Lactiplantibacillus plantarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Lactiplantibacillus plantarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | A9J246 | NC8 | - |
| Lactiplantibacillus plantarum NC8 | A9J246 | NC8 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant wild type and mutant enzymes | Lactiplantibacillus plantarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 263.4 | - |
after purification to homogeneity | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Lactiplantibacillus plantarum | D-tagatose | - |
? | |
| D-galactose | - |
Lactiplantibacillus plantarum NC8 | D-tagatose | - |
? | |
| L-arabinose | - |
Lactiplantibacillus plantarum | L-ribulose | - |
? | |
| L-arabinose | - |
Lactiplantibacillus plantarum NC8 | L-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | - |
Lactiplantibacillus plantarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-galactose isomerase | - |
Lactiplantibacillus plantarum |
| L-AI NC8 | - |
Lactiplantibacillus plantarum |
| L-arabinose aldose-ketose-isomerase | - |
Lactiplantibacillus plantarum |
| L-arabinose isomerase | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Lactiplantibacillus plantarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 65 | - |
Lactiplantibacillus plantarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable for 2 h in the presence of Mn2+ and Co2+ | Lactiplantibacillus plantarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Lactiplantibacillus plantarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Lactiplantibacillus plantarum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | at 25°C for 50 min | Lactiplantibacillus plantarum |
| 5 | - |
for 24 h | Lactiplantibacillus plantarum |
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