Application | Comment | Organism |
---|---|---|
nutrition | the product D-tagatose is used as a low-calorie bulk sweetener | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | enhances activity | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | Geobacillus stearothermophilus | - |
D-tagatose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
recombinant enzyme, expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
partially | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | - |
Geobacillus stearothermophilus | D-tagatose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-arabinose isomerase | mutant Gali 152 | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
free cells | Geobacillus stearothermophilus |
70 | - |
immobilized cells | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half life for tagatose production in free cells: 8.7 h, in immobilized cells: 632 h | Geobacillus stearothermophilus |
60 | - |
half life for tagatose production in free cells: 2.8 h, in immobilized cells: 144 h | Geobacillus stearothermophilus |
65 | - |
half life for tagatose production in free cells: 1.4 h, in immobilized cells: 26.0 h | Geobacillus stearothermophilus |
70 | - |
half life for tagatose production in free cells: 0.6 h, in immobilized cells: 12.5 h | Geobacillus stearothermophilus |
80 | - |
half life for tagatose production in free cells: 0.25 h, in immobilized cells: 7.0 h | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
immobilized cells | Geobacillus stearothermophilus |
7.5 | - |
free cells | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
half life for tagatose production in free cells: 1.0 h | Geobacillus stearothermophilus |
6 | - |
half life for tagatose production in free cells: 2.7 h, in immobilized cells: 71.4 h | Geobacillus stearothermophilus |
7 | - |
half life for tagatose production in free cells: 3.0 h, in immobilized cells: 150 h | Geobacillus stearothermophilus |
8 | - |
half life for tagatose production in free cells: 2.8 h, in immobilized cells: 144 h | Geobacillus stearothermophilus |
9 | - |
half life for tagatose production in free cells: 1.3 h, in immobilized cells: 25.9 h | Geobacillus stearothermophilus |