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Literature summary for 5.3.1.4 extracted from

  • Jung, E.S.; Kim, H.J.; Oh, D.K.
    Tagatose production by immobilized recombinant Escherichia coli cells containing Geobacillus stearothermophilus l-arabinose isomerase mutant in a packed-bed bioreactor (2005), Biotechnol. Prog., 21, 1335-1340.
    View publication on PubMed

Application

Application Comment Organism
nutrition the product D-tagatose is used as a low-calorie bulk sweetener Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ enhances activity Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose Geobacillus stearothermophilus
-
D-tagatose
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
recombinant enzyme, expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
partially Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
Geobacillus stearothermophilus D-tagatose
-
?

Synonyms

Synonyms Comment Organism
L-arabinose isomerase mutant Gali 152 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
free cells Geobacillus stearothermophilus
70
-
immobilized cells Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half life for tagatose production in free cells: 8.7 h, in immobilized cells: 632 h Geobacillus stearothermophilus
60
-
half life for tagatose production in free cells: 2.8 h, in immobilized cells: 144 h Geobacillus stearothermophilus
65
-
half life for tagatose production in free cells: 1.4 h, in immobilized cells: 26.0 h Geobacillus stearothermophilus
70
-
half life for tagatose production in free cells: 0.6 h, in immobilized cells: 12.5 h Geobacillus stearothermophilus
80
-
half life for tagatose production in free cells: 0.25 h, in immobilized cells: 7.0 h Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
immobilized cells Geobacillus stearothermophilus
7.5
-
free cells Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
half life for tagatose production in free cells: 1.0 h Geobacillus stearothermophilus
6
-
half life for tagatose production in free cells: 2.7 h, in immobilized cells: 71.4 h Geobacillus stearothermophilus
7
-
half life for tagatose production in free cells: 3.0 h, in immobilized cells: 150 h Geobacillus stearothermophilus
8
-
half life for tagatose production in free cells: 2.8 h, in immobilized cells: 144 h Geobacillus stearothermophilus
9
-
half life for tagatose production in free cells: 1.3 h, in immobilized cells: 25.9 h Geobacillus stearothermophilus