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Literature summary for 5.3.1.33 extracted from

  • Barbier, T.; Collard, F.; Zuniga-Ripa, A.; Moriyon, I.; Godard, T.; Becker, J.; Wittmann, C.; Van Schaftingen, E.; Letesson, J.J.
    Erythritol feeds the pentose phosphate pathway via three new isomerases leading to D-erythrose-4-phosphate in Brucella (2014), Proc. Natl. Acad. Sci. USA, 111, 17815-17820.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
tpiA2 is inserted into a pET15b vector, which allows expression of N-terminal His-tagged proteins in Escherichia coli Bl21pLysS Brucella abortus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-erythrulose 1-phosphate Brucella abortus the enzyme participates in erythritol catabolism D-erythrulose 4-phosphate
-
?
L-erythrulose 1-phosphate Brucella abortus 2308 the enzyme participates in erythritol catabolism D-erythrulose 4-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Brucella abortus Q2YIQ6
-
-
Brucella abortus 2308 Q2YIQ6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brucella abortus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-erythrulose 1-phosphate
-
Brucella abortus D-erythrulose 4-phosphate
-
?
L-erythrulose 1-phosphate the enzyme participates in erythritol catabolism Brucella abortus D-erythrulose 4-phosphate
-
?
L-erythrulose 1-phosphate
-
Brucella abortus 2308 D-erythrulose 4-phosphate
-
?
L-erythrulose 1-phosphate the enzyme participates in erythritol catabolism Brucella abortus 2308 D-erythrulose 4-phosphate
-
?

Synonyms

Synonyms Comment Organism
D-3-tetrulose-4-phosphate isomerase
-
Brucella abortus
eryH
-
Brucella abortus
TpiA2
-
Brucella abortus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Brucella abortus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Brucella abortus

General Information

General Information Comment Organism
malfunction the enzyme participates in erythritol catabolism. Erythritol is an important nutrient for Brucella, a worldwide pathogen that finds this four-carbon polyol in genital tissues Brucella abortus