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Literature summary for 5.3.1.3 extracted from
- Properties of D-arabinose isomerase purified from two strains of Escherichia coli (1973), J. Bacteriol., 113, 687-696.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Escherichia coli |  |
| Dulcitol | - |
Escherichia coli | |
| L-arabitol | - |
Escherichia coli | |
| ribitol | - |
Escherichia coli | |
| sorbitol | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 42 | - |
L-fucose | E. coli B/r | Escherichia coli | |
| 45 | - |
L-fucose | E. coli K-12 | Escherichia coli | |
| 170 | - |
D-arabinose | E. coli B/r | Escherichia coli | |
| 280 | - |
D-arabinose | E. coli K-12 | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates | Escherichia coli | |
| Mn2+ | stimulates | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84600 | - |
4 * 84600, Escherichia coli B/r, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea | Escherichia coli |
| 90900 | - |
4 * 90900, Escherichia coli K-12, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea | Escherichia coli |
| 342000 | - |
strain B/r, high-speed equilibrium sedimentation | Escherichia coli |
| 355000 | - |
strain K-12, high-speed equilibrium sedimentation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B/r | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:arabinose-grown cell | L-fucose induced culture of strain K-12 and D-arabinose-induced culture of strain B/r | Escherichia coli | - |
| culture condition:D-arabinose-induced cell | strain B/r | Escherichia coli | - |
| culture condition:L-fucose-induced cell | strain K-12 | Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 63.3 | - |
Escherichia coli K-12 | Escherichia coli |
| 63.7 | - |
Escherichia coli B/r | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose | - |
Escherichia coli | D-ribulose | - |
? | |
| D-arabinose | - |
Escherichia coli B/r | D-ribulose | - |
? | |
| L-Fucose | - |
Escherichia coli | L-Fuculose | - |
? | |
| L-Fucose | - |
Escherichia coli B/r | L-Fuculose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 84600, Escherichia coli B/r, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea | Escherichia coli |
| tetramer | 4 * 90900, Escherichia coli K-12, high speed sedimentation of enzyme dissociated into subunits by protonation at pH 2 or dialysis against buffer containing 8 M urea | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | 7.0: sharp decrease in activity below, 8.0-10.0: maximal activity | Escherichia coli |
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