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Literature summary for 5.3.1.27 extracted from

  • Orita, I.; Yurimoto, H.; Hirai, R.; Kawarabayasi, Y.; Sakai, Y.; Kato, N.
    The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathway (2005), J. Bacteriol., 187, 3636-3642.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
additional information expression of the full-length gene encoding the hybrid enzyme 3-hexulose 6-phosphate synthase/6-phospho-3-hexuloisomerase, the sequence corresponding to the 3-hexulose 6-phosphate synthase region, and the sequence corresponding to the 6-phospho-3-hexuloisomerase region produces active enzymes in Escherichia coli. The bifunctional enzyme catalyzes the sequential reaction much more efficiently than a mixture of the isolated domains Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
4 * 47000, SDS-PAGE, bifunctional enzyme, 4 * 21000, SDS-PAGE, and 4 * 21475, calculated, separately expressed 6-phospho-3-hexuloisomerase domain Pyrococcus horikoshii
21475
-
4 * 47000, SDS-PAGE, bifunctional enzyme, 4 * 21000, SDS-PAGE, and 4 * 21475, calculated, separately expressed 6-phospho-3-hexuloisomerase domain Pyrococcus horikoshii
47000
-
4 * 47000, SDS-PAGE, bifunctional enzyme, 4 * 21000, SDS-PAGE, and 4 * 21475, calculated, separately expressed 6-phospho-3-hexuloisomerase domain Pyrococcus horikoshii
75000
-
separately expressed 6-phospho-3-hexuloisomerase domain Pyrococcus horikoshii
162000
-
gel filtration, bifunctional enzyme Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O59601 bifunctional 3-hexulose 6-phosphate synthase/6-phospho-3-hexuloisomerase, constitutive expression
-

Subunits

Subunits Comment Organism
tetramer 4 * 47000, SDS-PAGE, bifunctional enzyme, 4 * 21000, SDS-PAGE, and 4 * 21475, calculated, separately expressed 6-phospho-3-hexuloisomerase domain Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
separately expressed 6-phospho-3-hexuloisomerase domain Pyrococcus horikoshii
80 85 bifunctional enzyme Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life above 90 min, bifunctional enzyme. Half-life below 5 min, separately expressed 6-phospho-3-hexuloisomerase domain Pyrococcus horikoshii