Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of the full-length gene encoding the hybrid enzyme 3-hexulose 6-phosphate synthase/6-phospho-3-hexuloisomerase, the sequence corresponding to the 3-hexulose 6-phosphate synthase region, and the sequence corresponding to the 6-phospho-3-hexuloisomerase region produces active enzymes in Escherichia coli. The bifunctional enzyme catalyzes the sequential reaction much more efficiently than a mixture of the isolated domains | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
4 * 47000, SDS-PAGE, bifunctional enzyme, 4 * 21000, SDS-PAGE, and 4 * 21475, calculated, separately expressed 6-phospho-3-hexuloisomerase domain | Pyrococcus horikoshii |
21475 | - |
4 * 47000, SDS-PAGE, bifunctional enzyme, 4 * 21000, SDS-PAGE, and 4 * 21475, calculated, separately expressed 6-phospho-3-hexuloisomerase domain | Pyrococcus horikoshii |
47000 | - |
4 * 47000, SDS-PAGE, bifunctional enzyme, 4 * 21000, SDS-PAGE, and 4 * 21475, calculated, separately expressed 6-phospho-3-hexuloisomerase domain | Pyrococcus horikoshii |
75000 | - |
separately expressed 6-phospho-3-hexuloisomerase domain | Pyrococcus horikoshii |
162000 | - |
gel filtration, bifunctional enzyme | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O59601 | bifunctional 3-hexulose 6-phosphate synthase/6-phospho-3-hexuloisomerase, constitutive expression | - |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 47000, SDS-PAGE, bifunctional enzyme, 4 * 21000, SDS-PAGE, and 4 * 21475, calculated, separately expressed 6-phospho-3-hexuloisomerase domain | Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
separately expressed 6-phospho-3-hexuloisomerase domain | Pyrococcus horikoshii |
80 | 85 | bifunctional enzyme | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life above 90 min, bifunctional enzyme. Half-life below 5 min, separately expressed 6-phospho-3-hexuloisomerase domain | Pyrococcus horikoshii |