BRENDA - Enzyme Database
show all sequences of 5.3.1.27

Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus

Ferenci, T.; Stroem, T.; Quayle, J.R.; Biochem. J. 144, 477-486 (1974)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
1 mM, 35% residual activity
Methylococcus capsulatus
Cu2+
1 mM, 8% residual activity
Methylococcus capsulatus
Mg2+
5 mM, 30% loss of activity
Methylococcus capsulatus
additional information
at 1 mm sugar phosphate concentration, D-allulose 6-phosphate, D-fructose 6-phosphate, 6-phospho-D-gluconate, D-ribulose 5-phosphate, D-xylulose 5-phosphate, D-erythrose 4-phosphate and glyceraldehyde 3-phosphate do not affect the isomerase activity
Methylococcus capsulatus
Ni2+
1 mM, 14% residual activity
Methylococcus capsulatus
Zn2+
1 mM, 40% residual activity
Methylococcus capsulatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
D-arabino-3-hexulose 6-phosphate
30C, pH 7.0
Methylococcus capsulatus
1.1
-
D-fructose 6-phosphate
30C, pH 7.0
Methylococcus capsulatus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for divalent cations
Methylococcus capsulatus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
gel filtration
Methylococcus capsulatus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methylococcus capsulatus
-
grown on methane
-
Purification (Commentary)
Commentary
Organism
-
Methylococcus capsulatus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1560
-
30C, pH 7.0
Methylococcus capsulatus
Storage Stability
Storage Stability
Organism
-15C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months
Methylococcus capsulatus
0-4C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months
Methylococcus capsulatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabino-3-hexulose 6-phosphate
-
684946
Methylococcus capsulatus
D-fructose 6-phosphate
-
-
-
r
D-fructose 6-phosphate
-
684946
Methylococcus capsulatus
D-arabino-3-hexulose 6-phosphate
-
-
-
r
additional information
enzyme is specific for substrates D-arabino-3-hexulose 6-phosphate and D-fructose 6-phosphate
684946
Methylococcus capsulatus
?
-
-
-
-
additional information
no substrate: D-ribulose 5-phosphate, D-xylulose 5-phosphate or D-allulose 6-phosphate
684946
Methylococcus capsulatus
?
-
-
-
-
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
10 min, complete loss of activity
Methylococcus capsulatus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Methylococcus capsulatus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
1 mM, 35% residual activity
Methylococcus capsulatus
Cu2+
1 mM, 8% residual activity
Methylococcus capsulatus
Mg2+
5 mM, 30% loss of activity
Methylococcus capsulatus
additional information
at 1 mm sugar phosphate concentration, D-allulose 6-phosphate, D-fructose 6-phosphate, 6-phospho-D-gluconate, D-ribulose 5-phosphate, D-xylulose 5-phosphate, D-erythrose 4-phosphate and glyceraldehyde 3-phosphate do not affect the isomerase activity
Methylococcus capsulatus
Ni2+
1 mM, 14% residual activity
Methylococcus capsulatus
Zn2+
1 mM, 40% residual activity
Methylococcus capsulatus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
D-arabino-3-hexulose 6-phosphate
30C, pH 7.0
Methylococcus capsulatus
1.1
-
D-fructose 6-phosphate
30C, pH 7.0
Methylococcus capsulatus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no requirement for divalent cations
Methylococcus capsulatus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
gel filtration
Methylococcus capsulatus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methylococcus capsulatus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1560
-
30C, pH 7.0
Methylococcus capsulatus
Storage Stability (protein specific)
Storage Stability
Organism
-15C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months
Methylococcus capsulatus
0-4C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months
Methylococcus capsulatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabino-3-hexulose 6-phosphate
-
684946
Methylococcus capsulatus
D-fructose 6-phosphate
-
-
-
r
D-fructose 6-phosphate
-
684946
Methylococcus capsulatus
D-arabino-3-hexulose 6-phosphate
-
-
-
r
additional information
enzyme is specific for substrates D-arabino-3-hexulose 6-phosphate and D-fructose 6-phosphate
684946
Methylococcus capsulatus
?
-
-
-
-
additional information
no substrate: D-ribulose 5-phosphate, D-xylulose 5-phosphate or D-allulose 6-phosphate
684946
Methylococcus capsulatus
?
-
-
-
-
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
10 min, complete loss of activity
Methylococcus capsulatus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Methylococcus capsulatus
Other publictions for EC 5.3.1.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747047
Rozova
Characterization of two recom ...
Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum
Biochemistry (Moscow)
82
176-185
2017
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-
1
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2
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4
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1
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2
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-
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1
2
-
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746827
Witthoff
Metabolic engineering of Cory ...
Bacillus subtilis 168, Bacillus subtilis
Appl. Environ. Microbiol.
81
2215-2225
2015
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1
1
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-
-
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-
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7
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1
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746885
Lessmeier
Production of carbon-13-label ...
Bacillus subtilis 168, Bacillus subtilis
Appl. Microbiol. Biotechnol.
99
10163-10176
2015
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1
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-
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6
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714569
Song
Overexpression of an HPS/PHI f ...
Mycobacterium gastri, Mycobacterium gastri MB19
Biotechnol. Lett.
32
1541-1548
2010
-
-
1
-
-
-
-
-
-
-
-
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7
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2
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1
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2
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701850
Yurimoto
Genomic organization and bioch ...
Bacillus methanolicus, Bacillus subtilis, Brevibacillus brevis, Methanosarcina sp., Methylobacillus flagellatus KT, Methylococcus capsulatus, Methylomonas aminofaciens, Mycobacterium gastri, Pyrococcus horikoshii, Pyrococcus sp., Thermococcus kodakarensis, Thermococcus sp.
Appl. Microbiol. Biotechnol.
84
407-416
2009
-
-
-
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-
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-
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16
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13
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16
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16
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16
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13
13
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684615
Orita
Bifunctional enzyme fusion of ...
Mycobacterium gastri, Mycobacterium gastri MB19
Appl. Microbiol. Biotechnol.
76
439-445
2007
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1
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3
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5
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2
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2
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1
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1
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3
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2
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2
2
1
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687371
Orita
The ribulose monophosphate pat ...
Thermococcus kodakarensis
J. Bacteriol.
188
4698-4704
2006
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1
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1
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1
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4
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1
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1
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1
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1
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687367
Orita
The archaeon Pyrococcus horiko ...
Pyrococcus horikoshii
J. Bacteriol.
187
3636-3642
2005
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1
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1
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5
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6
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1
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5
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1
2
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1
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690085
Martinez-Cruz
Crystal structure of MJ1247 pr ...
Methanocaldococcus jannaschii
Structure
10
195-204
2002
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1
1
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-
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2
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684155
Taylor
Cloning, purification and char ...
Bacillus subtilis
Acta Crystallogr. Sect. D
D57
1138-1140
2001
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1
1
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1
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687363
Yasueda
Bacillus subtilis yckG and yck ...
Bacillus subtilis
J. Bacteriol.
181
7154-7160
1999
2
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1
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3
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685642
Yanase
Production of D-[1-13C]fructos ...
Methylomonas aminofaciens, Methylomonas aminofaciens 77a
Biosci. Biotechnol. Biochem.
56
541-542
1992
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1
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684259
Kato
-
Properties of 3-hexulose phosp ...
no activity in Hansenula polymorpha, no activity in Hansenula polymorpha DL-1, no activity in Kloeckera sp., no activity in Kloeckera sp. 2201, uncultured bacterium, uncultured bacterium 77a
Agric. Biol. Chem.
41
1133-1140
1977
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2
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6
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1
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1
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1
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684946
Ferenci
Purification and properties of ...
Methylococcus capsulatus
Biochem. J.
144
477-486
1974
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6
2
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1
1
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