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Literature summary for 5.3.1.24 extracted from

  • Reisinger, B.; Bocola, M.; List, F.; Claren, J.; Rajendran, C.; Sterner, R.
    A sugar isomerization reaction established on various (betaalpha)8-barrel scaffolds is based on substrate-assisted catalysis (2012), Protein Eng. Des. Sel., 25, 751-760.
    View publication on PubMed
Search for more literature for 5.3.1.24

Protein Variants

Protein Variants Comment Organism
D127V the mutant shows strongly reduced activity compared to the wild type enzyme Thermotoga maritima
D127V/D169V the mutant shows strongly reduced activity compared to the wild type enzyme Thermotoga maritima
D127V/D173V the mutant shows strongly reduced activity compared to the wild type enzyme Thermotoga maritima
D130V the mutant shows strongly reduced activity compared to the wild type enzyme Thermotoga maritima
D130V/D176V the mutant shows strongly reduced activity compared to the wild type enzyme Thermotoga maritima
D169V the mutant shows strongly reduced activity compared to the wild type enzyme Thermotoga maritima
D173V the mutant shows strongly reduced activity compared to the wild type enzyme Thermotoga maritima
D176V inactive Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00028
-
 N-(5-phospho-beta-D-ribosyl)anthranilate wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.018
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D130V/D176V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.019
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V/D173V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.036
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V/D169V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.036
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D169V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.053
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D173V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.074
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.074
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D130V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate Thermotoga maritima
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
phosphoribosylanthranilate isomerase
-
 Thermotoga maritima
PRA isomerase
-
 Thermotoga maritima
TrpF
-
 Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000183
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D130V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.000283
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D173V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.0012
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D130V/D176V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.009
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.028
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D169V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.032
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V/D173V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.522
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V/D169V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
3.7
-
 N-(5-phospho-beta-D-ribosyl)anthranilate wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.018
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D130V/D176V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.019
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V/D173V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.036
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V/D169V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.036
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D169V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.053
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D173V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.074
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D127V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
0.074
-
 N-(5-phospho-beta-D-ribosyl)anthranilate mutant enzyme D130V, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima
13000
-
 N-(5-phospho-beta-D-ribosyl)anthranilate wild type enzyme, in 50 mM HEPES, pH 7.5, at 25°C Thermotoga maritima