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Literature summary for 5.3.1.24 extracted from

  • Ochoa-Leyva, A.; Soberon, X.; Sanchez, F.; Argueello, M.; Montero-Moran, G.; Saab-Rincon, G.
    Protein design through systematic catalytic loop exchange in the (beta/alpha)8 fold (2009), J. Mol. Biol., 387, 949-964.
    View publication on PubMed
Search for more literature for 5.3.1.24

Application

Application Comment Organism
biotechnology the work provides a method of exchanging variably sized loops within the (beta/alpha)8 fold, affording a novel starting point for the screening of novel activities as well as modest diversions from an original activity Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
the expression of PRAI-LoxP variants is performed in Escherichia coli Rosetta 2 cells transformed with pET28b+ plasmids containing the encoding sequences Escherichia coli

Protein Variants

Protein Variants Comment Organism
Mut_L2_FBPA loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 2, sequence of loop SNGGASFIAGKGVKSDVPQ, fructose-bisphosphate aldolase, EC 4.1.2.13 Escherichia coli
Mut_L2_MR loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 2, sequence of loop GYPAL, mandelate racemase, EC 5.1.2.2 Escherichia coli
Mut_L2_PRAI_WT loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 2, sequence of loop VATSPRCVN, phosphoribosylanthranilate isomerase, EC 5.3.1.24 Escherichia coli
Mut_L2_Ure loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 2, sequence of loop GGTGPAAGTHATTCTPG, urease, EC 3.5.1.5 Escherichia coli
Mut_L4_ADA loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop GDELGFPGSLF, adenosine diaminase, EC 3.5.4.4 Escherichia coli
Mut_L4_alphaTS loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop DVPVQQS, tryptophan synthase, EC 4.2.1.20 Escherichia coli
Mut_L4_DHDPS loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop PYYNRPS, dihydrodipicolinate synthase, EC 4.2.1.52 Escherichia coli
Mut_L4_FBPA loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop DLSEES, fructose-bisphosphate aldolase, EC 4.1.2.13 Escherichia coli
Mut_L4_MR loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop EPTLEHD, mandelate racemase, EC 5.1.2.2 Escherichia coli
Mut_L4_PBGS loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop AAMDG, porphobilinogen synthase, EC 4.2.1.24 Escherichia coli
Mut_L4_PRAI_WT loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop GNEE, phosphoribosylanthranilate isomerase, EC 5.3.1.24 Escherichia coli
Mut_L4_TPS loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop LGQEDLH, thiamine-phosphate diphosphorylase, EC 2.5.1.3 Escherichia coli
Mut_L4_Ure loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 4, sequence of loop EDWGAT, urease, EC 3.5.1.5 Escherichia coli
Mut_L6_alphaTS loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 6, sequence of loop SRAGVTGAENRAALP, tryptophan synthase, EC 4.2.1.20 Escherichia coli
Mut_L6_DHDPS loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 6, sequence of loop TGNL, dihydrodipicolinate synthase, EC 4.2.1.52 Escherichia coli
Mut_L6_PBGS loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 6, sequence of loop PAGAY, porphobilinogen synthase, EC 4.2.1.24 Escherichia coli
Mut_L6_PRAI_WT loop exchange in the PRAI-LoxP scaffold, position beta/alpha loop 6, sequence of loop NGQGGSGQRFD, phosphoribosylanthranilate isomerase, EC 5.3.1.24 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22900
-
 PRAI-LoxP, His-tag, apparent molecular mass, protein concentration 2.0 microM Escherichia coli
22900
-
 PRAI-LoxP, His-tag, apparent molecular mass, protein concentration 20 microM Escherichia coli
24100
-
 PRAI-LoxP, theoretical Escherichia coli
49800
-
 PRAI-LoxP, His-tag, apparent molecular mass, protein concentration 100 microM, deduced association state dimer Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P00909
-
-

Purification (Commentary)

Purification (Comment) Organism
a nickel Sepharose column, HisTrap FF, and a gel-filtration column, Sephacryl S200, are used Escherichia coli

Subunits

Subunits Comment Organism
monomer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
phosphoribosylanthranilate isomerase
-
 Escherichia coli
PRAI
-
 Escherichia coli
PRAI-LoxP variant of phosphoribosylanthranilate isomerase with an insertion coding for a Cre-lox recognition site in the loop linking alpha-helix 4 and beta-stand 5 Escherichia coli