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Literature summary for 5.3.1.24 extracted from

  • Eberhard, M.; Tsai-Pflugfelder, M.; Bolewska, K.; Hommel, U.; Kirschner, K.
    Indoleglycerol phosphate synthase - phosphoribosyl anthranilate isomerase: comparison of the bifunctional enzyme from Escherichia coli with engineered monofunctional domains (1995), Biochemistry, 34, 5419-5428.
    View publication on PubMed
Search for more literature for 5.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
separation of the 2 domains of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex on the gene level, expression of monofunctional PRAI[ML256-452] in Escherichia coli W3110 trpR, deltatrp EA2 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate product inhibition Escherichia coli
additional information not inhibited by indoleglycerol phosphate Escherichia coli
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate competitive inhibitor Escherichia coli
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate competitive inhibitor Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
 N-(5-phospho-beta-D-ribosyl)anthranilate 25°C, monomeric domain PRAI[ML256-452] Escherichia coli
0.0049
-
 N-(5-phospho-beta-D-ribosyl)anthranilate 25°C, bifunctional IGPS:PRAI Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
-
 Saccharomyces cerevisiae
23000
-
 monomeric domain PRAI[ML256-452], gel filtration and analytical ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate Saccharomyces cerevisiae involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate Saccharomyces cerevisiae xPRAI involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
Saccharomyces cerevisiae
-
 xPRAI
-
Saccharomyces cerevisiae xPRAI
-
 xPRAI
-

Purification (Commentary)

Purification (Comment) Organism
monomeric domain PRAI[ML256-452] and bifunctional IGPS:PRAI Escherichia coli
recombinant xPRAI, expressed in Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
98.7
-
 monofunctional domain PRAI[ML256-452] Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Saccharomyces cerevisiae 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Saccharomyces cerevisiae 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Saccharomyces cerevisiae xPRAI 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Saccharomyces cerevisiae xPRAI 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PRAI[ML256-452] engineered monofunctional variant of IGPS:PRAI, excised monomeric domain Escherichia coli
PRAI[ML256-452] engineered monofunctional variant of IGPS:PRAI, excised monomeric domain Saccharomyces cerevisiae
xPRAI monomeric variant of PRAI from Saccharomyces cerevisiae expressed in Escherichia coli Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32
-
 N-(5-phospho-beta-D-ribosyl)anthranilate 25°C, monomeric domain PRAI[ML256-452] Escherichia coli
40
-
 N-(5-phospho-beta-D-ribosyl)anthranilate 25°C Escherichia coli
40
-
 N-(5-phospho-beta-D-ribosyl)anthranilate bifunctional IGPS:PRAI Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0022
-
 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate recombinant xPRAI, expressed in Escherichia coli Saccharomyces cerevisiae
0.0065
-
 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, bifunctional IGPS:PRAI Escherichia coli
0.0068
-
 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, monomeric domain PRAI[ML256-452] Escherichia coli