BRENDA - Enzyme Database
show all sequences of 5.3.1.22

Purification and some properties of hydroxypyruvate isomerase of Bacillus fastidiosus

De Windt, F.E.; van der Drift, C.; Biochim. Biophys. Acta 613, 556-562 (1980)

Data extracted from this reference:

General Stability
General Stability
Organism
loss of activity by vacuum dialysis at 4C or by freezing
Bacillus fastidiosus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
0.1 mM, 57% inhibition
Bacillus fastidiosus
Fe2+
0.1 mM, 18% inhibition
Bacillus fastidiosus
hydroxylamine
20 mM, 60% inhibition
Bacillus fastidiosus
iodoacetate
0.7 mM, 100% inhibition
Bacillus fastidiosus
NaCN
2.5 mM, 21% inhibition
Bacillus fastidiosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
10
-
Hydroxypyruvate
-
Bacillus fastidiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
6 * 45000, SDS-PAGE
Bacillus fastidiosus
265000
-
gel filtration
Bacillus fastidiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus fastidiosus
-
strain DSM 83
-
Purification (Commentary)
Commentary
Organism
-
Bacillus fastidiosus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3125
-
-
Bacillus fastidiosus
Storage Stability
Storage Stability
Organism
4C, stable for 1 month
Bacillus fastidiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Hydroxypyruvate
r
2658
Bacillus fastidiosus
2-Hydroxy-3-oxopropanoate
r
2658
Bacillus fastidiosus
-
Subunits
Subunits
Commentary
Organism
hexamer
6 * 45000, SDS-PAGE
Bacillus fastidiosus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
57
-
-
Bacillus fastidiosus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
67
-
10 min stable
Bacillus fastidiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
7.4
-
Bacillus fastidiosus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.4
8.5
50% of maximal activity at pH 5.4 and 8.5
Bacillus fastidiosus
General Stability (protein specific)
General Stability
Organism
loss of activity by vacuum dialysis at 4C or by freezing
Bacillus fastidiosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
0.1 mM, 57% inhibition
Bacillus fastidiosus
Fe2+
0.1 mM, 18% inhibition
Bacillus fastidiosus
hydroxylamine
20 mM, 60% inhibition
Bacillus fastidiosus
iodoacetate
0.7 mM, 100% inhibition
Bacillus fastidiosus
NaCN
2.5 mM, 21% inhibition
Bacillus fastidiosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
10
-
Hydroxypyruvate
-
Bacillus fastidiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
6 * 45000, SDS-PAGE
Bacillus fastidiosus
265000
-
gel filtration
Bacillus fastidiosus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus fastidiosus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3125
-
-
Bacillus fastidiosus
Storage Stability (protein specific)
Storage Stability
Organism
4C, stable for 1 month
Bacillus fastidiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Hydroxypyruvate
r
2658
Bacillus fastidiosus
2-Hydroxy-3-oxopropanoate
r
2658
Bacillus fastidiosus
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 45000, SDS-PAGE
Bacillus fastidiosus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
57
-
-
Bacillus fastidiosus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
67
-
10 min stable
Bacillus fastidiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
7.4
-
Bacillus fastidiosus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.4
8.5
50% of maximal activity at pH 5.4 and 8.5
Bacillus fastidiosus
Other publictions for EC 5.3.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746832
Navone
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1
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4
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2
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1
1
1
1
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727247
Carvalho
An engineered pathway for glyo ...
Escherichia coli
BMC Biotechnol.
11
111
2011
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1
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3
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1
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1
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1
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2658
De Windt
Purification and some properti ...
Bacillus fastidiosus
Biochim. Biophys. Acta
613
556-562
1980
-
-
-
-
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1
5
1
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-
2
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-
2
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1
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-
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1
1
1
1
1
-
1
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1
1
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1
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5
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1
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2
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1
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1
1
1
1
1
-
1
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1
1
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