Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Serratia proteamaculans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Serratia proteamaculans | |
EDTA | - |
Serratia proteamaculans | |
Fe2+ | - |
Serratia proteamaculans | |
Ni2+ | - |
Serratia proteamaculans | |
Zn2+ | - |
Serratia proteamaculans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.83 | - |
D-xylulose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
13.3 | - |
D-Lyxose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
19.4 | - |
D-fructose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
32.2 | - |
D-mannose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the addition of Ca2+ ion to EDTA-treated enzyme enhances the activity of D-lyxose isomerization | Serratia proteamaculans | |
Mn2+ | maximum activity (190%) in the presence of 1 mM Mn2+ | Serratia proteamaculans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26756 | - |
2 * 26756, calculated from amino acid sequence | Serratia proteamaculans |
27000 | - |
2 * 27000, SDS-PAGE | Serratia proteamaculans |
54000 | - |
gel filtration | Serratia proteamaculans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia proteamaculans | D5MTT1 | - |
- |
Serratia proteamaculans KCTC 2936 | D5MTT1 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Serratia proteamaculans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
using L-sorbose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
0.14 | - |
using L-ribose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
0.19 | - |
using D-talose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
0.28 | - |
using D-tagatose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
0.4 | - |
using L-ribulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
1.05 | - |
using L-gulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
1.41 | - |
using D-fructose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
5.42 | - |
using D-mannose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
10 | - |
using D-lyxose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
26.2 | - |
using D-xylulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Serratia proteamaculans | D-mannose | - |
r | |
D-Lyxose | highest activity | Serratia proteamaculans | D-Xylulose | - |
r | |
D-Lyxose | highest activity | Serratia proteamaculans KCTC 2936 | D-Xylulose | - |
r | |
D-Mannose | - |
Serratia proteamaculans | D-Fructose | - |
r | |
D-Mannose | - |
Serratia proteamaculans KCTC 2936 | D-Fructose | - |
r | |
D-talose | - |
Serratia proteamaculans | D-tagatose | - |
r | |
D-talose | - |
Serratia proteamaculans KCTC 2936 | D-tagatose | - |
r | |
D-xylulose | - |
Serratia proteamaculans | D-lyxose | - |
r | |
L-gulose | - |
Serratia proteamaculans | L-sorbose | - |
r | |
L-ribose | - |
Serratia proteamaculans | L-ribulose | - |
r | |
L-ribose | - |
Serratia proteamaculans KCTC 2936 | L-ribulose | - |
r | |
additional information | no activity with D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate | Serratia proteamaculans | ? | - |
? | |
additional information | no activity with D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate | Serratia proteamaculans KCTC 2936 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 27000, SDS-PAGE | Serratia proteamaculans |
homodimer | 2 * 26756, calculated from amino acid sequence | Serratia proteamaculans |
Synonyms | Comment | Organism |
---|---|---|
D-Lyxose isomerase | - |
Serratia proteamaculans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Serratia proteamaculans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme shows half-lives of 84 h, 17 h, 2.6 h, 0.3 h, and 0.09 h at 30°C, 35°C, 40°C, 45°C, and 50°C, respectively | Serratia proteamaculans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4297 | - |
D-fructose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
16170 | - |
D-mannose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
30810 | - |
D-Lyxose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
31620 | - |
D-xylulose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Serratia proteamaculans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
222 | - |
D-fructose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
502 | - |
D-mannose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
2333 | - |
D-Lyxose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans | |
9560 | - |
D-xylulose | at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ | Serratia proteamaculans |