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Literature summary for 5.3.1.15 extracted from

  • Anderson, R.L.
    D-Lyxose isomerase (1966), Methods Enzymol., 9, 593-596.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
PCMB
-
Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
mannose
-
Klebsiella aerogenes
3.6
-
D-Lyxose
-
Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ metal ion required, Mn2+ activates most effectively. Km: 0.0004 mM Klebsiella aerogenes
Fe2+ metal ion required, Fe2+ activates Klebsiella aerogenes
Fe3+ metal ion required, Fe3+ activates Klebsiella aerogenes
Ca2+ metal ion required, Ca2+ activates slightly Klebsiella aerogenes
Co2+ metal ion required, Co2+ activates slightly Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration, density gradient centrifugation Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-lyxose-grown cell
-
Klebsiella aerogenes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.7
-
-
Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Lyxose
-
Klebsiella aerogenes D-Xylulose
-
?
D-Mannose
-
Klebsiella aerogenes D-Fructose
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Klebsiella aerogenes

pH Range

pH Minimum pH Maximum Comment Organism
4.9 7 pH 4.0: about 50% of maximal activity, pH 7.0: maximal activity Klebsiella aerogenes