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Literature summary for 5.3.1.14 extracted from
- Characterization of L-rhamnose isomerase from Clostridium stercorarium and its application to the production of D-allose from D-allulose (D-psicose) (2018), Biotechnol. Lett., 40, 325-334 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | under optimized conditions of pH 7, 70°C, 1 mM Mn2+, 27 U enzyme/l, and 600 g D-allulose/l, 199 g D-allose/l is produced without byproducts over 2.5 h, with a conversion yield of 33% and a productivity of 79.6 g/l/h | Thermoclostridium stercorarium subsp. stercorarium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1 mM, optimum concentration, 7fold increase in activity | Thermoclostridium stercorarium subsp. stercorarium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 154000 | - |
gel filtration | Thermoclostridium stercorarium subsp. stercorarium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoclostridium stercorarium subsp. stercorarium | L7VQ35 | - |
- |
| Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | L7VQ35 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-allose | - |
Thermoclostridium stercorarium subsp. stercorarium | D-psicose | - |
r | |
| D-allose | - |
Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | D-psicose | - |
r | |
| D-allulose | best ketose substrate | Thermoclostridium stercorarium subsp. stercorarium | D-psicose | - |
r | |
| D-ribose | - |
Thermoclostridium stercorarium subsp. stercorarium | D-ribulose | - |
r | |
| D-ribose | - |
Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | D-ribulose | - |
r | |
| D-ribulose | - |
Thermoclostridium stercorarium subsp. stercorarium | D-ribose | - |
r | |
| L-fructose | - |
Thermoclostridium stercorarium subsp. stercorarium | L-mannose | - |
r | |
| L-lyxose | - |
Thermoclostridium stercorarium subsp. stercorarium | L-xylulose | - |
r | |
| L-lyxose | - |
Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | L-xylulose | - |
r | |
| L-Mannose | - |
Thermoclostridium stercorarium subsp. stercorarium | L-Fructose | - |
r | |
| L-Mannose | - |
Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | L-Fructose | - |
r | |
| L-Rhamnose | best aldose substrate | Thermoclostridium stercorarium subsp. stercorarium | L-Rhamnulose | - |
r | |
| L-Rhamnose | best aldose substrate | Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | L-Rhamnulose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 49000, SDS-PAGE, 3 * 48998, calculated from sequence | Thermoclostridium stercorarium subsp. stercorarium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermoclostridium stercorarium subsp. stercorarium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
half-life 22.8 h | Thermoclostridium stercorarium subsp. stercorarium |
| 75 | - |
half-life 1.9 h | Thermoclostridium stercorarium subsp. stercorarium |
| 80 | - |
half-life 0.2 h | Thermoclostridium stercorarium subsp. stercorarium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermoclostridium stercorarium subsp. stercorarium |
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