Crystallization (Comment) | Organism |
---|---|
wild type and mutant enzymes S329F, S329K, S329L, and S329A in complex with L-rhamnose, D-psicose or D-allose, droplet vapor diffusion method, using 6-9.25% (w/v) polyethylene glycol 20000, 50 mM MES buffer, pH 6.1-6.3, at 20°C | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
S329A | mutant showing reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas stutzeri |
S329F | mutant showing reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas stutzeri |
S329K | mutant showing reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas stutzeri |
S329L | mutant showing reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.9 | - |
L-rhamnose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
12.2 | - |
L-rhamnose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
18.5 | - |
L-rhamnose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
19.4 | - |
L-rhamnose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
24.2 | - |
L-rhamnose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
25.9 | - |
D-allose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
33.9 | - |
D-allose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
35.6 | - |
D-allose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
39.1 | - |
D-ribose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
44.4 | - |
D-ribose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
44.7 | - |
D-allose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
53.3 | - |
D-allose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
65.6 | - |
D-ribose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
67.3 | - |
D-ribose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
85.1 | - |
D-ribose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | Q75WH8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Pseudomonas stutzeri | D-psicose | - |
r | |
D-ribose | - |
Pseudomonas stutzeri | D-ribulose | - |
r | |
L-Rhamnose | - |
Pseudomonas stutzeri | L-Rhamnulose | - |
r | |
additional information | the enzyme recognizes substrates at C2 and C3 but not at C4 and C5 | Pseudomonas stutzeri | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-RhI | - |
Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
D-ribose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
1.8 | - |
D-ribose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
2.7 | - |
D-allose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
3.3 | - |
D-ribose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
3.8 | - |
D-allose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.1 | - |
L-rhamnose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
7.4 | - |
D-allose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
8.9 | - |
D-ribose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
9.8 | - |
D-allose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
11.4 | - |
D-ribose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
13.3 | - |
L-rhamnose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
21.2 | - |
D-allose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
53.6 | - |
L-rhamnose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
142 | - |
L-rhamnose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
224 | - |
L-rhamnose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
D-ribose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.0043 | - |
D-ribose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.005 | - |
D-ribose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.071 | - |
D-allose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.081 | - |
D-allose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.13 | - |
D-ribose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.2 | - |
D-ribose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.27 | - |
D-allose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.28 | - |
D-allose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.41 | - |
L-rhamnose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.47 | - |
D-allose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
0.55 | - |
L-rhamnose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
6.8 | - |
L-rhamnose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
7.7 | - |
L-rhamnose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
11.6 | - |
L-rhamnose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri |