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Literature summary for 5.3.1.14 extracted from

  • Poonperm, W.; Takata, G.; Okada, H.; Morimoto, K.; Granstroem, T.B.; Izumori, K.
    Cloning, sequencing, overexpression and characterization of L-rhamnose isomerase from Bacillus pallidus Y25 for rare sugar production (2007), Appl. Microbiol. Biotechnol., 76, 1297-1307.
    View publication on PubMed
Search for more literature for 5.3.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene L-rhi, DNA and amino acid sequence determination and analysis, expression of the soluble His6-tagged enzyme in Escherichia coli strain JM109 Aeribacillus pallidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.89
-
 L-rhamnose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus
16.1
-
 L-Mannose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus
28.9
-
 L-Lyxose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus
34.9
-
 D-ribose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus
41.8
-
 D-allose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ strictly dependent on Mn2+ or Co2+, activates Aeribacillus pallidus
Mn2+ strictly dependent on Mn2+ or Co2+, activates at 1 mM Aeribacillus pallidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47636, sequence calculation, x * 47000, SDS-PAGE, native enzyme Aeribacillus pallidus
47636
-
 x * 47636, sequence calculation, x * 47000, SDS-PAGE, native enzyme Aeribacillus pallidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Rhamnose Aeribacillus pallidus
-
 L-Rhamnulose
-
 r
additional information Aeribacillus pallidus the enzyme generally occurs in the metabolism of fructose and mannose ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aeribacillus pallidus A8CEF6 fragment; i.e. Geobacillus pallidus, a facultative thermophilic bacterium, strain Y25, gene L-rhi
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange, hydrophobic interaction, and again anion exchange chromatography to apparent homogeneity, recombinant His6-tagged enzyme 3.87fold from Escherichia coli strain JM109 by nickel affinity chromatography to homogeneity Aeribacillus pallidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aeribacillus pallidus
2.58
-
 purified recombinant His-tagged enzyme, substrate D-allose Aeribacillus pallidus
4.52
-
 purified recombinant His-tagged enzyme, substrate L-mannose Aeribacillus pallidus
6.33
-
 purified recombinant His-tagged enzyme, substrate D-ribose Aeribacillus pallidus
19.3
-
 purified recombinant His-tagged enzyme, substrate L-lyxose Aeribacillus pallidus
77.2
-
 purified recombinant His-tagged enzyme, substrate L-rhamnose Aeribacillus pallidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose 35% conversion at 50°C Aeribacillus pallidus D-psicose
-
 r
D-gulose
-
 Aeribacillus pallidus D-sorbose
-
 r
D-ribose 16% conversion at 50°C Aeribacillus pallidus D-ribulose
-
 r
L-lyxose
-
 Aeribacillus pallidus L-xylulose
-
 r
L-Mannose 25% conversion at 50°C Aeribacillus pallidus L-Fructose
-
 r
L-Rhamnose
-
 Aeribacillus pallidus L-Rhamnulose
-
 r
L-tagatose 10% conversion at 50°C Aeribacillus pallidus L-talose
-
 r
additional information substrate specificity, overview Aeribacillus pallidus ?
-
 ?
additional information the enzyme generally occurs in the metabolism of fructose and mannose Aeribacillus pallidus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47636, sequence calculation, x * 47000, SDS-PAGE, native enzyme Aeribacillus pallidus

Synonyms

Synonyms Comment Organism
L-RhI
-
 Aeribacillus pallidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 recombinant enzyme Aeribacillus pallidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 65
-
 Aeribacillus pallidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 60 min, purified recombinant enzyme, pH 7.0, 90% remaining activity Aeribacillus pallidus
65
-
 60 min, purified recombinant enzyme, pH 7.0, 50% remaining activity Aeribacillus pallidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.2
-
 D-allose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus
31
-
 L-Mannose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus
34.5
-
 D-ribose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus
68
-
 L-rhamnose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus
76.8
-
 L-Lyxose pH 7.0, 65°C, recombinant enzyme Aeribacillus pallidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant enzyme Aeribacillus pallidus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 purified recombinant enzyme, stable Aeribacillus pallidus