KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55 | - |
L-rhamnose | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 30% of the activation compared to Mn2+ | Pseudomonas sp. | |
Mg2+ | 6% of the activiation compared to Mn2+ | Pseudomonas sp. | |
Mn2+ | activates | Pseudomonas sp. | |
Mn2+ | 1 mM MnCl2 enhances activity 4fold | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Pseudomonas sp. |
150000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. | - |
L-lyxose-utilizing mutant that produces L-rhamnose isomerase constitutively | - |
Pseudomonas sp. LL172 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
109 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | 10% of the activity relative to L-rhamnose | Pseudomonas sp. | D-psicose | - |
? | |
D-Gulose | 10% of the activity relative to L-rhamnose | Pseudomonas sp. | ? | - |
? | |
D-ribose | equilibrium ratio of D-ribose:D-ribulose is 70:30 | Pseudomonas sp. | D-ribulose | - |
? | |
D-ribose | 16% of the activity relative to L-rhamnose | Pseudomonas sp. | D-ribulose | - |
? | |
L-lyxose | equilibrium ratio of L-lyxose:L-xylulose is 70:30 | Pseudomonas sp. | L-xylulose | - |
? | |
L-lyxose | 97% of the activity relative to L-rhamnose | Pseudomonas sp. | L-xylulose | - |
? | |
L-lyxose | equilibrium ratio of L-lyxose:L-xylulose is 70:30 | Pseudomonas sp. LL172 | L-xylulose | - |
? | |
L-lyxose | 97% of the activity relative to L-rhamnose | Pseudomonas sp. LL172 | L-xylulose | - |
? | |
L-Mannose | 30% of the activity relative to L-rhamnose | Pseudomonas sp. | L-Fructose | - |
? | |
L-Mannose | equilibrium ratio of L-mannose:L-fructose is 30:70 | Pseudomonas sp. | L-Fructose | - |
? | |
L-Mannose | 30% of the activity relative to L-rhamnose | Pseudomonas sp. LL172 | L-Fructose | - |
? | |
L-Mannose | equilibrium ratio of L-mannose:L-fructose is 30:70 | Pseudomonas sp. LL172 | L-Fructose | - |
? | |
L-Rhamnose | - |
Pseudomonas sp. | L-Rhamnulose | - |
? | |
L-Rhamnose | - |
Pseudomonas sp. LL172 | L-Rhamnulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 9.0, 10 min | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 9.0, 10 min, stable | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
50°C | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 4°C, 24 h, stable | Pseudomonas sp. |
7 | 11 | 4°C, 24 h, pH 7: about 30% loss of activity, pH 9: stable, pH 11: about 10% loss of activity, free enzyme | Pseudomonas sp. |