Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BB-8 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme K59A, sitting drop vapor diffusion method, using 20% PEG 8K and 0.1 M HEPES, pH 7.5 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H88A | the enzyme site-specific mutant is able to catalyze the isomerization of A5P to R5P in vitro with 9.5% of the wild type protein activity | Escherichia coli |
K59A | catalytically inactive | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
full-length protein, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P45395 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelate affinity column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.55 | - |
pH and temperature not specified in the publication | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | x-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
API | - |
Escherichia coli |
D-arabinose-5-phosphate isomerase | - |
Escherichia coli |
KdsD | - |
Escherichia coli |