Literature summary for 5.3.1.13 extracted from

Mosberg, J.A.; Yep, A.; Meredith, T.C.; Smith, S.; Wang, P.F.; Holler, T.P.; Mobley, H.L.; Woodard, R.W.
A unique arabinose 5-phosphate isomerase found within a genomic island associated with the uropathogenicity of Escherichia coli CFT073 (2011), J. Bacteriol., 193, 2981-2988.

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Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	EDTA treatment increases the activity of c3406 protein by approximately 2.5fold	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	-	Escherichia coli
Hg2+	-	Escherichia coli
Zn2+	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.7	-	D-ribulose 5-phosphate	pH 6.6, 37°C	Escherichia coli
1.92	-	D-arabinose 5-phosphate	pH 6.6, 37°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ba2+	stimulates activity	Escherichia coli
Ca2+	stimulates activity	Escherichia coli
Co2+	stimulates activity	Escherichia coli
Cu2+	stimulates activity	Escherichia coli
Fe2+	stimulates activity	Escherichia coli
Mg2+	stimulates activity	Escherichia coli
Mn2+	stimulates activity	Escherichia coli
Ni2+	stimulates activity	Escherichia coli
Zn2+	c3406 protein possesses 0.72 equivalent of Zn2+ per subunit	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20888	-	x * 20888, mass spectrometry	Escherichia coli
21000	-	x * 21000, SDS-PAGE	Escherichia coli
73000	-	gel filtration	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli CFT073	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and MonoQ column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Arabinose 5-phosphate	-	Escherichia coli	D-Ribulose 5-phosphate	-	r
D-Arabinose 5-phosphate	-	Escherichia coli CFT073	D-Ribulose 5-phosphate	-	r
D-ribulose 5-phosphate	-	Escherichia coli	D-arabinose 5-phosphate	-	r
D-ribulose 5-phosphate	-	Escherichia coli CFT073	D-arabinose 5-phosphate	-	r
additional information	D-ribose 5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose 6-phosphate, and D-glucosamine 6-phosphate are no substrates	Escherichia coli	?	-	?
additional information	D-ribose 5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose 6-phosphate, and D-glucosamine 6-phosphate are no substrates	Escherichia coli CFT073	?	-	?

Subunits

Subunits	Comment	Organism
trimer or tetramer	x * 21000, SDS-PAGE	Escherichia coli
trimer or tetramer	x * 20888, mass spectrometry	Escherichia coli

Synonyms

Synonyms	Comment	Organism
A5P isomerase	-	Escherichia coli
arabinose 5-phosphate isomerase	-	Escherichia coli
c3406 protein	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
10.5	-	D-ribulose 5-phosphate	pH 6.6, 37°C	Escherichia coli
16.8	-	D-arabinose 5-phosphate	pH 6.6, 37°C	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.6	-	-	Escherichia coli

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	7.5	only trace A5P isomerase activity observed below pH 5.5 or above pH 7.5	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	a c3406 deletion mutant is still able to compete with wild type CFT073 in a transurethral cochallenge in mice and cannot colonize the mouse kidney	Escherichia coli
physiological function	the presence of c3406 is not essential for a pathogenic phenotype	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
8.8	-	D-arabinose 5-phosphate	pH 6.6, 37°C	Escherichia coli
15	-	D-ribulose 5-phosphate	pH 6.6, 37°C	Escherichia coli

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Release 2023.1 (January 2023)