Cloned (Comment) | Organism |
---|---|
kdsD and gutQ, API is encoded by the two paralogous genes, which may substitute for each other, expression of His-tagged KdsD in Escherichia coli strain BL21(DE3), subcloning in strain DH5alpha | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | inhibits substrate binding by the enzyme in a reversible manner | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Arabinose 5-phosphate | Escherichia coli | - |
D-Ribulose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
API is encoded by two paralogous genes kdsD and gutQ, which may substitute for each other | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged KdsD from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-arabinose 5-phosphate | KdsD converts 3-deoxy-A5P to 3-deoxy-Ru5P, but the catalysis proceeds tenfold slower than for the natural substrate isomerization | Escherichia coli | 3-deoxy-D-glycero-pentulose 5-phosphate | - |
? | |
4-deoxy-D-arabinose 5-phosphate | KdsD converts 4-deoxy-A5P to 4-deoxy-Ru5P, but the catalysis proceeds slower than for the natural substrate isomerization | Escherichia coli | 4-deoxy-D-glycero-pentulose 5-phosphate | - |
? | |
D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | - |
? | |
additional information | KdsD substrate specificity, overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
API | - |
Escherichia coli |
GutQ | - |
Escherichia coli |
KdsD | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Escherichia coli |