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Literature summary for 5.3.1.13 extracted from
- Escherichia coli YrbH is a D-arabinose 5-phosphate isomerase (2003), J. Biol. Chem., 278, 32771-32777.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 0.01 mM, complete inhibition | Escherichia coli |  |
| Cu2+ | - |
Escherichia coli | |
| EDTA | - |
Escherichia coli | |
| Hg2+ | 0.01 mM, complete inhibition | Escherichia coli | |
| Ni2+ | - |
Escherichia coli | |
| Zn2+ | 0.01 mM, complete inhibition, IC50: 0.001-0.003 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
D-ribulose 5-phosphate | pH 8.5, 37°C | Escherichia coli | |
| 0.61 | - |
D-arabinose 5-phosphate | pH 8.5, 37°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
4 * 35000, SDS-PAGE | Escherichia coli |
| 122000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate | Escherichia coli | first enzyme in the biosynthesis of 3-deoxy-D-manno-octulosonate | D-arabinose 5-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 246 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, stable for at least 1 month | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate | - |
Escherichia coli | D-arabinose 5-phosphate | - |
r | |
| D-ribulose 5-phosphate | first enzyme in the biosynthesis of 3-deoxy-D-manno-octulosonate | Escherichia coli | D-arabinose 5-phosphate | - |
r | |
| additional information | DL-glyceraldehyde 3-phosphate and D-erythrose 4-phosphate do not serve as alternate substrates | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 35000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| API | - |
Escherichia coli |
| YrbH | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 157 | - |
D-arabinose 5-phosphate | pH 8.5, 37°C | Escherichia coli | |
| 255 | - |
D-ribulose 5-phosphate | pH 8.5, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: about 70% of maximal activity, pH 10.0: about 70% of maximal activity | Escherichia coli |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.001 | 0.003 | 0.01 mM, complete inhibition, IC50: 0.001-0.003 mM | Escherichia coli | Zn2+ | |
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