Literature summary for 5.3.1.1 extracted from

Dumont, S.; Bykova, N.; Pelletier, G.; Dorion, S.; Rivoal, J.
Cytosolic triosephosphate isomerase from Arabidopsis thaliana is reversibly modified by glutathione on cysteines 127 and 218 (2016), Front. Plant Sci., 7, 1942 .

Search for more literature for 5.3.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
C127S	loss of more than 97% of specific activity	Arabidopsis thaliana
C127S/C218S	loss of more than 97% of specific activity	Arabidopsis thaliana
C218S	loss of about 21% of specific activity. In presence of glutathione disulfide, mutant behaves similarly to the wild-type, but retains more activity than the wild-type after 240 min of incubation	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
Diamide	1 mM, presence of glutathione, complete inhibition by S-glutathionylation	Arabidopsis thaliana
glutathione	-	Arabidopsis thaliana
glutathione disulfide	modification of residues Cys127 and Cys218	Arabidopsis thaliana
H2O2	0.5 mM, 120 min, 30% loss of activity in presence and absence of glutathione	Arabidopsis thaliana
N-ethylmaleimide	0.5 mM, 60 min, more than 90% loss of activity. Addition of dithiothreitol does not restore activity. In the presence of 1 mM glutathione, the addition of 0.5 mM N-ethylmaleimide does not significantly affect activity	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.48	-	D-glyceraldehyde 3-phosphate	mutant C127S, pH 7.8, 30°C	Arabidopsis thaliana
0.5	-	D-glyceraldehyde 3-phosphate	mutant C127S/C218S, pH 7.8, 30°C	Arabidopsis thaliana
1.32	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.8, 30°C	Arabidopsis thaliana
1.68	-	D-glyceraldehyde 3-phosphate	mutant C218S, pH 7.8, 30°C	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Arabidopsis thaliana	5829	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	P48491	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Arabidopsis thaliana	dihydroxyacetone 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
CTIMC	-	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
14.8	-	D-glyceraldehyde 3-phosphate	mutant C127S/C218S, pH 7.8, 30°C	Arabidopsis thaliana
70.4	-	D-glyceraldehyde 3-phosphate	mutant C127S, pH 7.8, 30°C	Arabidopsis thaliana
2690	-	D-glyceraldehyde 3-phosphate	mutant C218S, pH 7.8, 30°C	Arabidopsis thaliana
3406	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.8, 30°C	Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
29.6	-	D-glyceraldehyde 3-phosphate	mutant C127S/C218S, pH 7.8, 30°C	Arabidopsis thaliana
147	-	D-glyceraldehyde 3-phosphate	mutant C127S, pH 7.8, 30°C	Arabidopsis thaliana
1601	-	D-glyceraldehyde 3-phosphate	mutant C218S, pH 7.8, 30°C	Arabidopsis thaliana
2580	-	D-glyceraldehyde 3-phosphate	wild-type, pH 7.8, 30°C	Arabidopsis thaliana

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Release 2023.1 (January 2023)