Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Chlamydomonas reinhardtii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using either 65% (v/v) 2-methyl-2,4-pentanediol 0.1 M HEPES, pH 7.5, or 6% (w/v) PEG 6000 0.1 M HEPES, pH 7.0, or 17.5% (w/v) PEG 3350, 0.1 M Bis-Tris, pH 5.5, or 15% (w/v) PEG 3350 0.1 M Bis-Tris, pH 5.0 | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | the inhibition of the enzyme is negligible when incubated with 0.1 mM H2O2, whereas a 10% inhibition is observed with 1 mM H2O2 | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.04 | - |
D-glyceraldehyde 3-phosphate | in 100 mM triethanolamine (pH 7.4), at 25°C | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28249 | - |
2 * 28249, calculated from amino acid sequence | Chlamydomonas reinhardtii |
28266 | - |
2 * 28266, MALDI-TOF mass spectrometry | Chlamydomonas reinhardtii |
58800 | - |
gel filtration | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | Chlamydomonas reinhardtii | - |
Glycerone phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | Q5S7Y5 | - |
- |
Oxidation Stability | Organism |
---|---|
the inhibition of the enzyme is negligible when incubated with 0.1 mM H2O2, whereas a 10% inhibition is observed with 1 mM H2O2 | Chlamydomonas reinhardtii |
Purification (Comment) | Organism |
---|---|
Ni2+ HiTrap chelating resin column chromatography | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7193 | - |
in 100 mM triethanolamine (pH 7.4), at 25°C | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Chlamydomonas reinhardtii | Glycerone phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28249, calculated from amino acid sequence | Chlamydomonas reinhardtii |
homodimer | 2 * 28266, MALDI-TOF mass spectrometry | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
TpI | - |
Chlamydomonas reinhardtii |
Triosephosphate isomerase | - |
Chlamydomonas reinhardtii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3372 | - |
D-glyceraldehyde 3-phosphate | in 100 mM triethanolamine (pH 7.4), at 25°C | Chlamydomonas reinhardtii |
General Information | Comment | Organism |
---|---|---|
metabolism | the chloroplastic isoform participates in the Calvin-Benson cycle | Chlamydomonas reinhardtii |