Literature summary for 5.3.1.1 extracted from

Moraes, J.; Arreola, R.; Cabrera, N.; Saramago, L.; Freitas, D.; Masuda, A.; da Silva Vaz, I.; Tuena de Gomez-Puyou, M.; Perez-Montfort, R.; Gomez-Puyou, A.; Logullo, C.
Structural and biochemical characterization of a recombinant triosephosphate isomerase from Rhipicephalus (Boophilus) microplus (2011), Insect Biochem. Mol. Biol., 41, 400-409.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS cells	Rhipicephalus microplus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.1 M HEPES-Na, pH 7.5, and 1.4 M trisodium citrate dihydrate	Rhipicephalus microplus

Inhibitors

Inhibitors	Comment	Organism	Structure
5,5'-dithio-bis(2-nitrobenzoic acid)	about 50% inhibition at about 0.05 mM	Rhipicephalus microplus
methyl methanethiosulfonate	about 50% inhibition at about 0.05 mM	Rhipicephalus microplus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.47	-	D-glyceraldehyde 3-phosphate	in 100 mM triethanolamine, pH 7.4, at 25°C	Rhipicephalus microplus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	2 * 27000, SDS-PAGE	Rhipicephalus microplus
27188	-	2 * 27188, calculated from amino acid sequence	Rhipicephalus microplus

Organism

Organism	UniProt	Comment	Textmining
Rhipicephalus microplus	A8B3A8	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Mono-Q column column chromatography	Rhipicephalus microplus

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Rhipicephalus microplus	-

Storage Stability

Storage Stability	Organism
4°C, purified recombinant enzyme, 40 days, no loss of activity	Rhipicephalus microplus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Rhipicephalus microplus	dihydroxyacetone phosphate	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 27000, SDS-PAGE	Rhipicephalus microplus
homodimer	2 * 27188, calculated from amino acid sequence	Rhipicephalus microplus

Synonyms

Synonyms	Comment	Organism
TIM	-	Rhipicephalus microplus
Triosephosphate isomerase	-	Rhipicephalus microplus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Rhipicephalus microplus	calculated from amino acid sequence	-	7.7

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Release 2023.1 (January 2023)