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Literature summary for 5.3.1.1 extracted from

  • Xu, Y.; Lorieau, J.; McDermott, A.E.
    Triosephosphate isomerase: 15N and 13C chemical shift assignments and conformational change upon ligand binding by magic-angle spinning solid-state NMR spectroscopy (2010), J. Mol. Biol., 397, 233-248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of TIM in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, 200 mg/ml protein with ligand in a ratio of 5:1 in 50 mM Tris-HCl, 50 mM NaCl, and 1 mM EDTA, pH 6.8, 4°C, is mixed with 40% w/v PEG 4000 as precipitant, X-ray diffraction structure determination and analysis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Glyceraldehyde 3-phosphate Saccharomyces cerevisiae
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Glycerone phosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00942
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant TIM from Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glyceraldehyde 3-phosphate
-
Saccharomyces cerevisiae Glycerone phosphate
-
?
D-Glyceraldehyde 3-phosphate substrate binding perturbs residues Asn10, His95, Ser100, Glu129-Glu133, Val167-Ala186, Asn213, and Leu230-Leu126 that either are near the ligand or have direct contact with the ligand Saccharomyces cerevisiae Glycerone phosphate
-
?

Subunits

Subunits Comment Organism
More determination of conformational dynamics of the enzyme, in solid state and in solution, with or without bound ligand, by NMR analysis, detailed overview Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
TIM
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Saccharomyces cerevisiae
Triosephosphate isomerase
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Saccharomyces cerevisiae