Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus woesei |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, diffraction quality can only be obtained in the presence of inhibitors, monoclinic crystal form is obtained by cocrystallization with 20 mM 2-carboxyethylphosphonic acid with sodium acetate, pH 4.0, and 7% PEG 4000 as the precipitant buffer. An orthorhombic crystal form is obtained by cocrystallization with 20 mM 2-phosphoglycolic acid with 0.1 M sodium acetate, pH 4.2, and 5% PEG 4000 as the precipitant buffer. Hexagonal crystal forms are obtained under the same conditions with 2-phosphoglycolic acid, but their diffraction shows high disorder | Pyrococcus woesei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-carboxyethylphosphonic acid | - |
Pyrococcus woesei | |
2-phosphoglycolic acid | - |
Pyrococcus woesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
4 * 24000, crystallographic data | Pyrococcus woesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus woesei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pyrococcus woesei |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 24000, crystallographic data | Pyrococcus woesei |