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Literature summary for 5.2.1.B4 extracted from

  • Rocco, C.J.; Wetterhorn, K.M.; Garvey, G.S.; Rayment, I.; Escalante-Semerena, J.C.
    The PrpF protein of Shewanella oneidensis MR-1 catalyzes the isomerization of 2-methyl-cis-aconitate during the catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle (2017), PLoS ONE, 12, e0188130 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.2.1.B4

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, high-resolution three-dimensional crystal structures of mutant enzyme K73E in complex with trans-aconitate or malonate provides insights into the mechanism of catalysis of the wild-type protein Shewanella oneidensis

Protein Variants

Protein Variants Comment Organism
C107A protein containing the C107A substitution is not overproduced and could not be purified Shewanella oneidensis
C107S inactive mutant enzyme Shewanella oneidensis
K73A protein containing the K73A substitution is not overproduced and could not be purified Shewanella oneidensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-4-methyl-cis-aconitate Shewanella oneidensis the enzyme is involved in catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle. The 2-methylcitric acid cycle is a common route of propionate catabolism in microorganisms 2-methyl-cis-aconitate
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 ?

Organism

Organism UniProt Comment Textmining
Shewanella oneidensis Q8EJW4
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-4-methyl-cis-aconitate the enzyme is involved in catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle. The 2-methylcitric acid cycle is a common route of propionate catabolism in microorganisms Shewanella oneidensis 2-methyl-cis-aconitate
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 ?
(R)-4-methyl-cis-aconitate
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 Shewanella oneidensis 2-methyl-cis-aconitate
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 ?

Synonyms

Synonyms Comment Organism
2-methyl-aconitate isomerase
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 Shewanella oneidensis
PrpF protein
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 Shewanella oneidensis

General Information

General Information Comment Organism
metabolism the enzyme is involved in catabolism of propionate via the AcnD-dependent 2-methylcitric acid cycle. The 2-methylcitric acid cycle is a common route of propionate catabolism in microorganisms Shewanella oneidensis