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Literature summary for 5.2.1.8 extracted from

  • Kramer, G.; Patzelt, H.; Rauch, T.; Kurz, T.A.; Vorderwulbecke, S.; Bukau, B.; Deuerling, E.
    Trigger factor's peptidyl-prolyl cis/trans isomerase activity is not essential for the folding of cytosolic proteins in Escherichia coli (2004), J. Biol. Chem., 279, 14165-14179.
    View publication on PubMed
Search for more literature for 5.2.1.8

Protein Variants

Protein Variants Comment Organism
E178V slightly more active than wild-type enzyme with succinyl-Ala-Phe-Pro-Phe-4-nitroanilide, as active as wild-type enzyme in refolding of reduced and carboxymethylated RNAse T1, chaperone activity comparable with wild-type, not impaired in association with nascent proteins Escherichia coli
F198A inactive with succinyl-Ala-Phe-Pro-Phe-4-nitroanilide, inactive in refolding of reduced and carboxymethylated RNAse T1, chaperone activity comparable with wild-type, not impaired in association with nascent proteins Escherichia coli
F233L inactive with succinyl-Ala-Phe-Pro-Phe-4-nitroanilide, less active than wild-type enzyme in refolding of reduced and carboxymethylated RNAse T1, chaperone activity comparable with wild-type, not impaired in association with nascent proteins Escherichia coli
Y221F mutant enzyme shows 15% of the activity of the wild-type enzyme with succinyl-Ala-Phe-Pro-Phe-4-nitroanilide as substrate, less active than wild-type enzyme in refolding of reduced and carboxymethylated RNAse T1, chaperone activity comparable with wild-type, not impaired in association with nascent proteins Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli trigger factor's peptidyl-prolyl cis/trans isomerase activity is not essential for the folding of cytosolic proteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information trigger factor's peptidyl-prolyl cis/trans isomerase activity is not essential for the folding of cytosolic proteins Escherichia coli ?
-
 ?
RNAse T1 reduced and carboxymethylated RNAse T1, refolding by trans to cis isomerization of peptidyl-prolyl bonds at Pro39 and Pro55 Escherichia coli ?
-
 ?
succinyl-Ala-Phe-(trans)-Pro-Phe-4-nitroanilide
-
 Escherichia coli succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide
-
 ?

Synonyms

Synonyms Comment Organism
TF
-
 Escherichia coli
trigger factor
-
 Escherichia coli