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Literature summary for 5.2.1.5 extracted from
- Linoleic acid isomerase in Lactobacillus plantarum AKU1009a proved to be a multi-component enzyme system requiring oxidoreduction cofactors (2011), Biosci. Biotechnol. Biochem., 75, 318-322.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 125% activity at 3 mM | Lactiplantibacillus plantarum |  |
| 8-hydroxyquinoline | 118% activity at 3 mM | Lactiplantibacillus plantarum | |
| alpha-alpha'-bipyridyl | 125% activity at 3 mM | Lactiplantibacillus plantarum | |
| FAD | the addition of 5 mM NADH plus FAD to the reaction mixture with dialyzed high molecular fraction of the soluble fraction restores activity to 101% | Lactiplantibacillus plantarum | |
| K2MoO4 | 422% activity at 5 mM, in the presence of o-phenanthroline | Lactiplantibacillus plantarum | |
| K2WO4 | 149% activity at 5 mM, in the presence of o-phenanthroline | Lactiplantibacillus plantarum | |
| Na2MnO4 | 427% activity at 5 mM, in the presence of o-phenanthroline | Lactiplantibacillus plantarum | |
| Na2WO4 | 157% activity at 5 mM, in the presence of o-phenanthroline | Lactiplantibacillus plantarum | |
| Na3VO4 | 264% activity at 5 mM, in the presence of o-phenanthroline | Lactiplantibacillus plantarum | |
| NADH | the addition of 5 mM NADH plus FAD to the reaction mixture with dialyzed high molecular fraction of the soluble fraction restores activity to 101% | Lactiplantibacillus plantarum | |
| NADPH | the addition of 5 mM NADPH to the reaction mixture with dialyzed high molecular fraction of the soluble fraction restores activity to 98% | Lactiplantibacillus plantarum | |
| NaVO3 | 209% activity at 5 mM, in the presence of o-phenanthroline | Lactiplantibacillus plantarum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 93% residual activity at 3 mM | Lactiplantibacillus plantarum | |
| EGTA | 93% residual activity at 3 mM | Lactiplantibacillus plantarum | |
| o-phenanthroline | 10% residual activity at 3 mM | Lactiplantibacillus plantarum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metal ions such as Co2+, Ni2+, Fe3+, Cd3+, and Mn2+ restore conjugated linoleic acid productivity after inhibition with o-phenanthroline up to 56% of the original activity | Lactiplantibacillus plantarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | - |
- |
- |
| Lactiplantibacillus plantarum AKU1009a | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ultracentrifugation | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9-cis,12-cis-linoleic acid | - |
Lactiplantibacillus plantarum | 9-cis,11-trans-linoleic acid + 9-trans,11-trans-linoleic acid | - |
? | |
| 9-cis,12-cis-linoleic acid | - |
Lactiplantibacillus plantarum AKU1009a | 9-cis,11-trans-linoleic acid + 9-trans,11-trans-linoleic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LA-I | - |
Lactiplantibacillus plantarum |
| Linoleic acid isomerase | - |
Lactiplantibacillus plantarum |
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Release 2023.1 (January 2023)
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