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Literature summary for 5.2.1.4 extracted from

  • Liu, T.T.; Zhou, N.Y.
    Novel L-cysteine-dependent maleylpyruvate isomerase in the gentisate pathway of Paenibacillus sp. strain NyZ101 (2012), J. Bacteriol., 194, 3987-3994.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene bagL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, comparison of genetic organization of gentisate catabolic gene clusters, recombinant expression of the N-terminall His6-tagged enzyme in Escherichia coli strain BL21(DE3) Paenibacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ni2+ activates Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-Maleylpyruvate Paenibacillus sp.
-
 3-Fumarylpyruvate
-
 ?
3-Maleylpyruvate Paenibacillus sp. NyZ101
-
 3-Fumarylpyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. H6TG59 gene bagL
-
Paenibacillus sp. NyZ101 H6TG59 gene bagL
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-Maleylpyruvate
-
 Paenibacillus sp. 3-Fumarylpyruvate
-
 ?
3-Maleylpyruvate
-
 Paenibacillus sp. NyZ101 3-Fumarylpyruvate
-
 ?
additional information BagL is a thiol-dependent maleylpyruvate isomerase catalyzing the isomerization of maleylpyruvate to fumarylpyruvate with L-cysteine, cysteinylglycine, or glutathione, as its cofactor Paenibacillus sp. ?
-
 ?
additional information BagL is a thiol-dependent maleylpyruvate isomerase catalyzing the isomerization of maleylpyruvate to fumarylpyruvate with L-cysteine, cysteinylglycine, or glutathione, as its cofactor Paenibacillus sp. NyZ101 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Paenibacillus sp.
More sequence analysis and homology modeling Paenibacillus sp.

Synonyms

Synonyms Comment Organism
BagL
-
 Paenibacillus sp.
thiol-dependent maleylpyruvate isomerase
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Paenibacillus sp.

Cofactor

Cofactor Comment Organism Structure
additional information L-cysteine, cysteinylglycine, or glutathione are possible cofactor, dependent on, cofactor kinetics, overview Paenibacillus sp.

Expression

Organism Comment Expression
Paenibacillus sp. the bag cluster is highly transcribed in 3-hydroxybenzoateinduced cells of strain NyZ101 up

General Information

General Information Comment Organism
evolution BagL is a L-cysteine-dependent catabolic enzyme in microbial metabolism and is distinct member of DinB family, with a four-helix-bundle topology Paenibacillus sp.