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Literature summary for 5.2.1.2 extracted from
- Maleylacetoacetate cis-trans isomerase (1989), Coenzymes and cofactors, Glutathione, Chem. Biochem. Med. Aspects Pt. A (Dolphin D, Poulson R, Avromonic O, eds. ) John Wiley & Sons, New York, 3, 733-751.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GSH | required as coenzyme | Vibrio sp. |  |
| GSH | other thiols are inactive | Vibrio sp. | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GSSG | - |
Vibrio sp. | |
| N-Hexylmaleamic acid | noncompetitive towards GSH and maleylacetone | Vibrio sp. | |
| NEM | - |
Vibrio sp. | |
| S-Glutathioyl-N-hexylmaleamate | competitive towards GSH, uncompetitive towards maleylacetone | Vibrio sp. | |
| S-Methylglutathione | - |
Vibrio sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
maleylacetone | - |
Vibrio sp. | |
| 1.9 | - |
6-Oxo-2(E),4(Z)-heptanedienoic acid | - |
Vibrio sp. | |
| 2.9 | - |
trans-beta-Acetylacrylate | - |
Vibrio sp. | |
| 3 | - |
cis-beta-Acetylacrylate | - |
Vibrio sp. | |
| 3.2 | - |
6-Oxo-2(Z),4(E)-heptanedienoic acid | - |
Vibrio sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
x * 16000, SDS-PAGE after heating the enzyme at 100°C for 10 min in a standard aqueous medium containing 1% SDS and 8M urea | Vibrio sp. |
| 35000 | - |
gel filtration | Vibrio sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Maleylacetoacetate | Vibrio sp. | one of the enzymes responsible for the oxidative metabolism of aromatic amino acids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mammalia | - |
- |
- |
| Vibrio sp. | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mammalia | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-Oxo-2(E),4(Z)-heptanedienoic acid | - |
Vibrio sp. | 6-Oxo-2(Z),4(E)-heptanedienoic acid | - |
? | |
| cis-beta-Acetylacrylate | - |
Vibrio sp. | trans-beta-Acetylacrylate | - |
? | |
| Maleylacetoacetate | - |
Vibrio sp. | Fumarylacetoacetate | - |
? | |
| Maleylacetoacetate | one of the enzymes responsible for the oxidative metabolism of aromatic amino acids | Vibrio sp. | ? | - |
? | |
| maleylacetone | - |
Vibrio sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Vibrio sp. |
| ? | x * 16000, SDS-PAGE after heating the enzyme at 100°C for 10 min in a standard aqueous medium containing 1% SDS and 8M urea | Vibrio sp. |
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