Cloned (Comment) | Organism |
---|---|
gene CoCRTISO1-ORa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
gene CoCRTISO1-ORb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
gene CoCRTISO1-Y, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
gene CoCRTISO2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
gene CoCRTISO3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
gene CoCRTISO4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
Protein Variants | Comment | Organism |
---|---|---|
A412S | site-directed mutagenesis, the mutant shows substrate specificity like the wild-type enzyme | Calendula officinalis |
G450E | site-directed mutagenesis, the isozyme CoCRTISO1-ORb mutant is catalytically inactive | Calendula officinalis |
I370T | site-directed mutagenesis, the isozyme CoCRTISO1-ORa mutant is catalytically inactive | Calendula officinalis |
L581P | site-directed mutagenesis, the isozyme CoCRTISO1-ORa mutant is catalytically inactive | Calendula officinalis |
P584L | site-directed mutagenesis, the mutant shows substrate specificity like the wild-type enzyme | Calendula officinalis |
S412A | site-directed mutagenesis, the isozyme CoCRTISO1-ORb mutant is catalytically inactive | Calendula officinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Calendula officinalis | isozyme substrate specificity in different genotypes, overview | ? | - |
? | |
additional information | Calendula officinalis | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color | ? | - |
? | |
additional information | Calendula officinalis | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color | ? | - |
? | |
additional information | Calendula officinalis | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Calendula officinalis | G9MAL3 | isozyme CoCRTISO1-ORa; gene crtiso | - |
Calendula officinalis | G9MAL4 | isozyme CoCRTISO1-ORb; gene crtiso | - |
Calendula officinalis | G9MAL5 | isozyme CoCRTISO1-Y; gene crtiso | - |
Calendula officinalis | G9MAL6 | isozyme CoCRTISO2; gene crtiso | - |
Calendula officinalis | G9MAL7 | isozyme CoCRTISO3; gene crtiso | - |
Calendula officinalis | G9MAL8 | isozyme CoCRTISO4; gene crtiso | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Calendula officinalis | - |
petal | - |
Calendula officinalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5'Z)-gamma-carotene | - |
Calendula officinalis | (all-E)-gamma-carotene | - |
? | |
(5'Z)-gamma-carotene | no activity with the wild-type, but with mutant 1-ORa462insCHH | Calendula officinalis | (all-E)-gamma-carotene | - |
? | |
(5'Z)-gamma-carotene | no activity with the wild-type, but with variant E450G | Calendula officinalis | (all-E)-gamma-carotene | - |
? | |
(5'Z)-gamma-carotene | wild-type and mutants I370T and A412S | Calendula officinalis | (all-E)-gamma-carotene | - |
? | |
(5'Z)-rubixanthin | no activity with the wild-type, but with mutant 1-ORa462insCHH | Calendula officinalis | (all-E)-rubixanthin | - |
? | |
(5'Z)-rubixanthin | no activity with the wild-type, but with variant E450G | Calendula officinalis | (all-E)-rubixanthin | - |
? | |
(5'Z)-rubixanthin | wild-type and mutants I370T and A412S | Calendula officinalis | (all-E)-rubixanthin | - |
? | |
(5Z,9Z,5'Z,9'Z)-lycopene | - |
Calendula officinalis | (all-E)-lycopene | - |
? | |
(5Z,9Z,5'Z,9'Z)-lycopene | no activity with the wild-type, but with mutant 1-ORa462insCHH | Calendula officinalis | (all-E)-lycopene | - |
? | |
(5Z,9Z,5'Z,9'Z)-lycopene | no activity with the wild-type, but with variant E450G | Calendula officinalis | (all-E)-lycopene | - |
? | |
(5Z,9Z,5'Z,9'Z)-lycopene | wild-type and mutants I370T and A412S | Calendula officinalis | (all-E)-lycopene | - |
? | |
additional information | isozyme substrate specificity in different genotypes, overview | Calendula officinalis | ? | - |
? | |
additional information | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color | Calendula officinalis | ? | - |
? | |
additional information | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color | Calendula officinalis | ? | - |
? | |
additional information | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration | Calendula officinalis | ? | - |
? | |
prolycopene | - |
Calendula officinalis | (all-E)-lycopene | - |
? | |
prolycopene | no activity with the wild-type, but with mutant 1-ORa462insCHH | Calendula officinalis | (all-E)-lycopene | - |
? | |
prolycopene | no activity with the wild-type, but with variant E450G | Calendula officinalis | (all-E)-lycopene | - |
? | |
prolycopene | wild-type and mutants I370T and A412S | Calendula officinalis | (all-E)-lycopene | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carotenoid isomerase | - |
Calendula officinalis |
CoCRTISO1-ORa | - |
Calendula officinalis |
CoCRTISO1-ORb | - |
Calendula officinalis |
CoCRTISO1Y | - |
Calendula officinalis |
CoCRTISO2 | - |
Calendula officinalis |
CoCRTISO3 | - |
Calendula officinalis |
CoCRTISO4 | - |
Calendula officinalis |
CRTISO | - |
Calendula officinalis |
General Information | Comment | Organism |
---|---|---|
malfunction | the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids | Calendula officinalis |
malfunction | the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. A Gly-to-Glu amino acid substitution at position 450 in CoCRTISO1-ORb abolishes enzyme activity completely | Calendula officinalis |
malfunction | the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. Deletion of Cys-His-His at positions 462-464 in isozyme CoCRTISO1-ORa abolishes enzyme activity completely | Calendula officinalis |
additional information | residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme | Calendula officinalis |
additional information | residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme | Calendula officinalis |
physiological function | isozyme CoCRTISO1 is a key regulator of the accumulation of 5-cis-carotenoids in calendula petals | Calendula officinalis |