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Literature summary for 5.2.1.13 extracted from

  • Kishimoto, S.; Ohmiya, A.
    Carotenoid isomerase is key determinant of petal color of Calendula officinalis (2012), J. Biol. Chem., 287, 276-285.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene CoCRTISO1-ORa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis Calendula officinalis
gene CoCRTISO1-ORb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis Calendula officinalis
gene CoCRTISO1-Y, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis Calendula officinalis
gene CoCRTISO2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis Calendula officinalis
gene CoCRTISO3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis Calendula officinalis
gene CoCRTISO4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis Calendula officinalis

Protein Variants

Protein Variants Comment Organism
A412S site-directed mutagenesis, the mutant shows substrate specificity like the wild-type enzyme Calendula officinalis
G450E site-directed mutagenesis, the isozyme CoCRTISO1-ORb mutant is catalytically inactive Calendula officinalis
I370T site-directed mutagenesis, the isozyme CoCRTISO1-ORa mutant is catalytically inactive Calendula officinalis
L581P site-directed mutagenesis, the isozyme CoCRTISO1-ORa mutant is catalytically inactive Calendula officinalis
P584L site-directed mutagenesis, the mutant shows substrate specificity like the wild-type enzyme Calendula officinalis
S412A site-directed mutagenesis, the isozyme CoCRTISO1-ORb mutant is catalytically inactive Calendula officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Calendula officinalis isozyme substrate specificity in different genotypes, overview ?
-
?
additional information Calendula officinalis isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color ?
-
?
additional information Calendula officinalis isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color ?
-
?
additional information Calendula officinalis isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration ?
-
?

Organism

Organism UniProt Comment Textmining
Calendula officinalis G9MAL3 isozyme CoCRTISO1-ORa; gene crtiso
-
Calendula officinalis G9MAL4 isozyme CoCRTISO1-ORb; gene crtiso
-
Calendula officinalis G9MAL5 isozyme CoCRTISO1-Y; gene crtiso
-
Calendula officinalis G9MAL6 isozyme CoCRTISO2; gene crtiso
-
Calendula officinalis G9MAL7 isozyme CoCRTISO3; gene crtiso
-
Calendula officinalis G9MAL8 isozyme CoCRTISO4; gene crtiso
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Calendula officinalis
-
petal
-
Calendula officinalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5'Z)-gamma-carotene
-
Calendula officinalis (all-E)-gamma-carotene
-
?
(5'Z)-gamma-carotene no activity with the wild-type, but with mutant 1-ORa462insCHH Calendula officinalis (all-E)-gamma-carotene
-
?
(5'Z)-gamma-carotene no activity with the wild-type, but with variant E450G Calendula officinalis (all-E)-gamma-carotene
-
?
(5'Z)-gamma-carotene wild-type and mutants I370T and A412S Calendula officinalis (all-E)-gamma-carotene
-
?
(5'Z)-rubixanthin no activity with the wild-type, but with mutant 1-ORa462insCHH Calendula officinalis (all-E)-rubixanthin
-
?
(5'Z)-rubixanthin no activity with the wild-type, but with variant E450G Calendula officinalis (all-E)-rubixanthin
-
?
(5'Z)-rubixanthin wild-type and mutants I370T and A412S Calendula officinalis (all-E)-rubixanthin
-
?
(5Z,9Z,5'Z,9'Z)-lycopene
-
Calendula officinalis (all-E)-lycopene
-
?
(5Z,9Z,5'Z,9'Z)-lycopene no activity with the wild-type, but with mutant 1-ORa462insCHH Calendula officinalis (all-E)-lycopene
-
?
(5Z,9Z,5'Z,9'Z)-lycopene no activity with the wild-type, but with variant E450G Calendula officinalis (all-E)-lycopene
-
?
(5Z,9Z,5'Z,9'Z)-lycopene wild-type and mutants I370T and A412S Calendula officinalis (all-E)-lycopene
-
?
additional information isozyme substrate specificity in different genotypes, overview Calendula officinalis ?
-
?
additional information isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color Calendula officinalis ?
-
?
additional information isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color Calendula officinalis ?
-
?
additional information isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration Calendula officinalis ?
-
?
prolycopene
-
Calendula officinalis (all-E)-lycopene
-
?
prolycopene no activity with the wild-type, but with mutant 1-ORa462insCHH Calendula officinalis (all-E)-lycopene
-
?
prolycopene no activity with the wild-type, but with variant E450G Calendula officinalis (all-E)-lycopene
-
?
prolycopene wild-type and mutants I370T and A412S Calendula officinalis (all-E)-lycopene
-
?

Synonyms

Synonyms Comment Organism
carotenoid isomerase
-
Calendula officinalis
CoCRTISO1-ORa
-
Calendula officinalis
CoCRTISO1-ORb
-
Calendula officinalis
CoCRTISO1Y
-
Calendula officinalis
CoCRTISO2
-
Calendula officinalis
CoCRTISO3
-
Calendula officinalis
CoCRTISO4
-
Calendula officinalis
CRTISO
-
Calendula officinalis

General Information

General Information Comment Organism
malfunction the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids Calendula officinalis
malfunction the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. A Gly-to-Glu amino acid substitution at position 450 in CoCRTISO1-ORb abolishes enzyme activity completely Calendula officinalis
malfunction the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. Deletion of Cys-His-His at positions 462-464 in isozyme CoCRTISO1-ORa abolishes enzyme activity completely Calendula officinalis
additional information residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme Calendula officinalis
additional information residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme Calendula officinalis
physiological function isozyme CoCRTISO1 is a key regulator of the accumulation of 5-cis-carotenoids in calendula petals Calendula officinalis