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Literature summary for 5.2.1.1 extracted from

  • Hatakeyama, K.; Goto, M.; Kobayashi, M.; Terasawa, M.; Yukawa, H.
    Analysis of oxidation sensitivity of maleate cis-trans isomerase from Serratia marcescens (2000), Biosci. Biotechnol. Biochem., 64, 1477-1485.
    View publication on PubMed
Search for more literature for 5.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Serratia marcescens

Protein Variants

Protein Variants Comment Organism
M138C mutant enzyme shows 90% of the activity of the wild-type enzyme Serratia marcescens
M18C mutant enzyme shows 30% of the activity of the wild-type enzyme Serratia marcescens
M201C mutant enzyme shows 90% of the activity of the wild-type enzyme Serratia marcescens
M46C mutant enzyme shows 30% of the activity of the wild-type enzyme Serratia marcescens
M57C mutant enzyme shows 40% of the activity of the wild-type enzyme Serratia marcescens
M85C mutant enzyme shows 90% of the activity of the wild-type enzyme Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
H2O2 1 mM, complete inhibition, Met201 is one of the targeted sites for the inactivation by chemical oxidation Serratia marcescens
iodoacetamide 1 mM, 72% inhibition Serratia marcescens
N-bromosuccinimide 1 mM, complete inhibition Serratia marcescens
N-Chlorosuccinimide 1 mM, complete inhibition Serratia marcescens
p-toluenesulfonchloramide 1 mM, complete inhibition Serratia marcescens
PCMB 0.1 mM, complete inhibition Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26950
-
 x * 26950, calculation from nucleotide sequence Serratia marcescens
28000
-
 x * 28000, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Geobacillus stearothermophilus
-
-
-
Serratia marcescens
-
 IFO3736
-
Serratia marcescens IFO3736
-
 IFO3736
-

Purification (Commentary)

Purification (Comment) Organism
-
 Serratia marcescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
-
 Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Maleate
-
 Alcaligenes faecalis Fumarate
-
 ?
Maleate
-
 Geobacillus stearothermophilus Fumarate
-
 ?
Maleate
-
 Serratia marcescens Fumarate
-
 ?
Maleate
-
 Serratia marcescens IFO3736 Fumarate
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Serratia marcescens
? x * 26950, calculation from nucleotide sequence Serratia marcescens

Synonyms

Synonyms Comment Organism
MaiA
-
 Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Alcaligenes faecalis
45
-
-
 Serratia marcescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 increase of activity from 30°C to 70°C Geobacillus stearothermophilus
30 60 30°C: about 60% of maximal activity, 60°C: about 40% of maximal activity Alcaligenes faecalis
30 60 30°C: about 70% of maximal activity, 60°C: about 70% of maximal activity Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, stable Alcaligenes faecalis
45
-
 30 min, about 10% loss of activity Alcaligenes faecalis
50
-
 30 min, about 45% loss of activity Alcaligenes faecalis
53
-
 30 min, complete inactivation Alcaligenes faecalis
57
-
 30 min, stable below Geobacillus stearothermophilus
60
-
 30 min, about 25% loss of activity Geobacillus stearothermophilus
60
-
 30 min, retains almost 60% of the initial activity Serratia marcescens