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Literature summary for 5.2.1.1 extracted from
- Studies on the induced synthesis of maleate cis-trans isomerase by malonate. Part III. Purification and properties of maleate cis-trans isomerase induced by malonate (1969), Agric. Biol. Chem., 33, 718-728.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Alcaligenes faecalis |  |
| cyanide | KCN, 1 mM, 20% inhibition | Alcaligenes faecalis | |
| EDTA | 1 mM, 7% inhibition | Alcaligenes faecalis | |
| Hg2+ | - |
Alcaligenes faecalis | |
| iodoacetate | 1 mM 73% inhibition | Alcaligenes faecalis | |
| PCMB | 0.1 mM, complete inhibition; inhibition is overcome by the addition of sulfhydryl compounds | Alcaligenes faecalis | |
| Sodium periodate | 1 mM, complete inhibition | Alcaligenes faecalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
Maleate | - |
Alcaligenes faecalis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alcaligenes faecalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alcaligenes faecalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Alcaligenes faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Maleate | reverse reaction is not observed | Alcaligenes faecalis | Fumarate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
15 min, stable below | Alcaligenes faecalis |
| 40 | - |
15 min, about 25% loss of activity | Alcaligenes faecalis |
| 50 | - |
15 min, complete loss of activity | Alcaligenes faecalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Alcaligenes faecalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.3 | pH 7: about 60% of maximal activity, pH 9.3: about 40% of maximal activity | Alcaligenes faecalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | stable over this range in presence of dithiothreitol for a few weeks. In absence of dithiothreitol the activity is markedly decreased, especially in the alkaline region | Alcaligenes faecalis |
| 6 | - |
most stable in acetate buffer at this pH | Alcaligenes faecalis |
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