Activating Compound | Comment | Organism | Structure |
---|---|---|---|
mercaptans | - |
Pseudomonas fluorescens | |
mercaptans | required for activity | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
General Stability | Organism |
---|---|
crystallization can be accompanied by a 50% loss in activity | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bromoacetate | - |
Pseudomonas fluorescens | |
Bromoacetate | - |
Pseudomonas sp. | |
cyanide | 1 mM, weak inhibition | Alcaligenes faecalis | |
deoxycholate | - |
Pseudomonas sp. | |
EDTA | - |
Alcaligenes faecalis | |
EDTA | 0.1 mM, 20% inhibition | Pseudomonas sp. | |
Fe2+ | - |
Alcaligenes faecalis | |
Hg2+ | - |
Alcaligenes faecalis | |
iodoacetate | - |
Alcaligenes faecalis | |
iodoacetate | 0.01 mM, complete inhibition | Pseudomonas sp. | |
N-bromosuccinimide | 1 mM, weak inhibition | Alcaligenes faecalis | |
NaCN | 0.6 mM, 20% inhibition | Pseudomonas sp. | |
NaF | 0.6-3 mM, 18-45% inhibition | Pseudomonas sp. | |
p-Substituted mercuribenzoate | - |
Alcaligenes faecalis | |
p-Substituted mercuribenzoate | 0.01 mM, complete inhibition | Pseudomonas sp. | |
Semicarbazide | 1 mM, weak inhibition | Alcaligenes faecalis | |
Sodium periodate | - |
Alcaligenes faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Maleate | - |
Pseudomonas sp. | |
2.8 | - |
Maleate | - |
Alcaligenes faecalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CO2 | increases activity by 20-30% | Alcaligenes faecalis | |
Fe2+ | increases activity by 20-30% | Alcaligenes faecalis | |
Mn2+ | increases activity by 20-30% | Alcaligenes faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
sedimentation velocity experiments | Pseudomonas fluorescens |
74000 | - |
sedimentation velocity experiments | Pseudomonas sp. |
100000 | - |
gel filtration, sedimentation velocity studies | Alcaligenes faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Alcaligenes faecalis | malonate and ketomalonate are more effective than maleate in inducing isomerase synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Maleate | - |
Alcaligenes faecalis | Fumarate | - |
? | |
Maleate | - |
Pseudomonas fluorescens | Fumarate | - |
? | |
Maleate | - |
Pseudomonas sp. | Fumarate | - |
? | |
additional information | malonate and ketomalonate are more effective than maleate in inducing isomerase synthesis | Alcaligenes faecalis | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
25 | pH 6, maximal stability in presence of small quantities of dithiothreitol | Alcaligenes faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
- |
Alcaligenes faecalis |
8.4 | - |
- |
Pseudomonas fluorescens |
8.4 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 9.4 | - |
Pseudomonas fluorescens |
6.7 | 9.4 | about 50% of maximal activity at pH 6.7 and pH 9.4 | Pseudomonas sp. |
6.8 | 9.3 | about 50% of maximal activity at pH 6.8 and 9.3 | Alcaligenes faecalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | 30°C, rapid loss of activity below pH 5 and above pH 7 | Alcaligenes faecalis |
6 | - |
0°C-25°C, maximal stability in presence of small quantities of dithiothreitol | Alcaligenes faecalis |