BRENDA - Enzyme Database show
show all sequences of 5.1.99.6

Plants utilize a highly conserved system for repair of NADH and NADPH hydrates

Niehaus, T.D.; Richardson, L.G.; Gidda, S.K.; Elbadawi-Sidhu, M.; Meissen, J.K.; Mullen, R.T.; Fiehn, O.; Hanson, A.D.; Plant Physiol. 165, 52-61 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of N-terminally His-tagged enzyme in Escherichia coli
Arabidopsis thaliana
expression of N-terminally His-tagged enzyme in Escherichia coli
Zea mays
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0006
-
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
pH and temperature not specified in the publication
Arabidopsis thaliana
0.00115
-
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
pH and temperature not specified in the publication
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
chloroplast
-
Zea mays
9507
-
cytoplasm
-
Arabidopsis thaliana
5737
-
cytoplasm
-
Zea mays
5737
-
mitochondrion
-
Arabidopsis thaliana
5739
-
mitochondrion
-
Zea mays
5739
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
Zea mays
i.e. NADHX
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
Arabidopsis thaliana
i.e. NADHX
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
Zea mays
i.e. NADPHX
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
Arabidopsis thaliana
i.e. NADPHX
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9LTX3
-
-
Zea mays
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Arabidopsis thaliana
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Zea mays
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
i.e. NADHX
728528
Zea mays
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
i.e. NADHX
728528
Arabidopsis thaliana
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
i.e. NADPHX
728528
Zea mays
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
i.e. NADPHX
728528
Arabidopsis thaliana
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
-
-
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.26
-
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
pH and temperature not specified in the publication
Arabidopsis thaliana
0.38
-
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
pH and temperature not specified in the publication
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the hydratase is dependent on ATP, but the epimerase ist not
Arabidopsis thaliana
additional information
the hydratase is dependent on ATP, but the epimerase ist not
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of N-terminally His-tagged enzyme in Escherichia coli
Arabidopsis thaliana
expression of N-terminally His-tagged enzyme in Escherichia coli
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the hydratase is dependent on ATP, but the epimerase ist not
Arabidopsis thaliana
additional information
the hydratase is dependent on ATP, but the epimerase ist not
Zea mays
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0006
-
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
pH and temperature not specified in the publication
Arabidopsis thaliana
0.00115
-
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
pH and temperature not specified in the publication
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
chloroplast
-
Zea mays
9507
-
cytoplasm
-
Arabidopsis thaliana
5737
-
cytoplasm
-
Zea mays
5737
-
mitochondrion
-
Arabidopsis thaliana
5739
-
mitochondrion
-
Zea mays
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
Zea mays
i.e. NADHX
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
Arabidopsis thaliana
i.e. NADHX
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
Zea mays
i.e. NADPHX
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
Arabidopsis thaliana
i.e. NADPHX
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Arabidopsis thaliana
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Zea mays
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
i.e. NADHX
728528
Zea mays
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
i.e. NADHX
728528
Arabidopsis thaliana
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
i.e. NADPHX
728528
Zea mays
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
-
-
r
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
i.e. NADPHX
728528
Arabidopsis thaliana
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.26
-
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
pH and temperature not specified in the publication
Arabidopsis thaliana
0.38
-
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
pH and temperature not specified in the publication
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
malfunction
a truncated form of the Arabidopsis epimerase lacking the PPOX domain is also fully active in the interconversion
Arabidopsis thaliana
additional information
the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family
Arabidopsis thaliana
additional information
the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family
Zea mays
physiological function
NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments
Arabidopsis thaliana
physiological function
NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments
Zea mays
General Information (protein specific)
General Information
Commentary
Organism
malfunction
a truncated form of the Arabidopsis epimerase lacking the PPOX domain is also fully active in the interconversion
Arabidopsis thaliana
additional information
the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family
Arabidopsis thaliana
additional information
the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family
Zea mays
physiological function
NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments
Arabidopsis thaliana
physiological function
NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments
Zea mays
Other publictions for EC 5.1.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747329
Niehaus
Evidence that the metabolite ...
Escherichia coli
Biosci. Rep.
38
BSR20180223
2018
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746703
Kremer
NAXE mutations disrupt the ce ...
Homo sapiens
Am. J. Hum. Genet.
99
894-902
2016
-
1
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726917
Marbaix
Occurrence and subcellular dis ...
Homo sapiens, Mus musculus, Rattus norvegicus
Biochem. J.
460
49-58
2014
-
-
1
-
-
-
-
-
6
-
-
6
-
3
-
-
1
-
-
3
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
6
-
-
-
1
-
3
-
-
6
-
-
-
-
-
3
-
-
-
-
3
3
-
-
-
728528
Niehaus
Plants utilize a highly conser ...
Arabidopsis thaliana, Zea mays
Plant Physiol.
165
52-61
2014
-
-
2
-
-
-
-
2
6
-
-
4
-
2
-
-
2
-
-
1
-
-
4
-
-
-
-
2
-
-
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
2
6
-
-
4
-
-
-
2
-
1
-
-
4
-
-
-
-
2
-
-
-
-
-
5
5
-
-
-
719988
Marbaix
Extremely conserved ATP- or AD ...
Mus musculus, Saccharomyces cerevisiae
J. Biol. Chem.
286
41246-41252
2011
4
-
2
-
-
-
-
2
-
-
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-
-
7
-
-
2
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
4
-
2
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-
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2
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-
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2
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-
-
-
4
-
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-
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2
-
-
-
-
-
-
-
-
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-