Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition | Pseudomonas sp. | |
DL-5-isopropylhydantoin | complete inhibition, pre-incubation with DL-5-(2-methylthioethyl)hydantoin protects | Pseudomonas sp. | |
Zn2+ | strong inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.8 | - |
D-5-(2-methylthioethyl)hydantoin | pH 7.5, 30°C | Pseudomonas sp. | |
79.7 | - |
L-5-(2-methylthioethyl)hydantoin | pH 7.5, 30°C | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight stimulation | Pseudomonas sp. | |
Mn2+ | slight stimulation | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Pseudomonas sp. |
190000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q00924 | - |
- |
Pseudomonas sp. NS671 | Q00924 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.8 | - |
pH 7.5, 30°C | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
4°C, ammonium sulfate at 90% saturation, stable for 6 months | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-5-isobutylhydantoin | complete racemization | Pseudomonas sp. | L-5-isobutylhydantoin | - |
r | |
D-5-isobutylhydantoin | complete racemization | Pseudomonas sp. NS671 | L-5-isobutylhydantoin | - |
r | |
D-5-methylhydantoin | complete racemization | Pseudomonas sp. | L-5-methylhydantoin | - |
r | |
D-5-methylhydantoin | complete racemization | Pseudomonas sp. NS671 | L-5-methylhydantoin | - |
r | |
L-5-(2-methylthioethyl)hydantoin | complete racemization | Pseudomonas sp. | D-5-(2-methylthioethyl)hydantoin | - |
r | |
L-5-(2-methylthioethyl)hydantoin | complete racemization | Pseudomonas sp. NS671 | D-5-(2-methylthioethyl)hydantoin | - |
r | |
L-5-methylhydantoin | about 50% racemization | Pseudomonas sp. | D-5-methylhydantoin | - |
r | |
L-5-methylhydantoin | about 50% racemization | Pseudomonas sp. NS671 | D-5-methylhydantoin | - |
r | |
additional information | no substrate: DL-5-isopropylhydantoin | Pseudomonas sp. | ? | - |
? | |
additional information | no substrate: DL-5-isopropylhydantoin | Pseudomonas sp. NS671 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | x * 32000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
in presence of substrate, stable | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
11 | - |
95% of maximum activity | Pseudomonas sp. |