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Literature summary for 5.1.99.5 extracted from

  • Watabe, K.; Ishikawa, T.; Mukohara, Y.; Nakamura, H.
    Purification and characterization of the hydantoin racemase of Pseudomonas sp. strain NS671 expressed in Escherichia coli (1992), J. Bacteriol., 174, 7989-7995.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition Pseudomonas sp.
DL-5-isopropylhydantoin complete inhibition, pre-incubation with DL-5-(2-methylthioethyl)hydantoin protects Pseudomonas sp.
Zn2+ strong inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
29.8
-
D-5-(2-methylthioethyl)hydantoin pH 7.5, 30°C Pseudomonas sp.
79.7
-
L-5-(2-methylthioethyl)hydantoin pH 7.5, 30°C Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight stimulation Pseudomonas sp.
Mn2+ slight stimulation Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Pseudomonas sp.
190000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q00924
-
-
Pseudomonas sp. NS671 Q00924
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.8
-
pH 7.5, 30°C Pseudomonas sp.

Storage Stability

Storage Stability Organism
4°C, ammonium sulfate at 90% saturation, stable for 6 months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-5-isobutylhydantoin complete racemization Pseudomonas sp. L-5-isobutylhydantoin
-
r
D-5-isobutylhydantoin complete racemization Pseudomonas sp. NS671 L-5-isobutylhydantoin
-
r
D-5-methylhydantoin complete racemization Pseudomonas sp. L-5-methylhydantoin
-
r
D-5-methylhydantoin complete racemization Pseudomonas sp. NS671 L-5-methylhydantoin
-
r
L-5-(2-methylthioethyl)hydantoin complete racemization Pseudomonas sp. D-5-(2-methylthioethyl)hydantoin
-
r
L-5-(2-methylthioethyl)hydantoin complete racemization Pseudomonas sp. NS671 D-5-(2-methylthioethyl)hydantoin
-
r
L-5-methylhydantoin about 50% racemization Pseudomonas sp. D-5-methylhydantoin
-
r
L-5-methylhydantoin about 50% racemization Pseudomonas sp. NS671 D-5-methylhydantoin
-
r
additional information no substrate: DL-5-isopropylhydantoin Pseudomonas sp. ?
-
?
additional information no substrate: DL-5-isopropylhydantoin Pseudomonas sp. NS671 ?
-
?

Subunits

Subunits Comment Organism
hexamer x * 32000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
in presence of substrate, stable Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
11
-
95% of maximum activity Pseudomonas sp.